Registration Dossier
Registration Dossier
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EC number: 207-434-1 | CAS number: 471-01-2
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP-study, according to interantional accepted guidelines. Data were taken from the structural analogue beta-Isophoron, CAS 78-59-1. The structural analogue differs in the location of the carbon double bound within the ring structure of the molecule, whereas the rest of the molecule is identical to CAS 471-01-2.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3,5,5-trimethylcyclohex-2-enone
- EC Number:
- 201-126-0
- EC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Cas Number:
- 78-59-1
- IUPAC Name:
- 3,5,5-trimethylcyclohex-2-en-1-one
- Details on test material:
- 3,5,5-trimethylcyclohexen-1-one,
Purity > 99.5 %
Source: Huels AG
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- INOCULUM/TEST ORGANISM
- Source: municipal WWVTP Marl-Ost, sampled 12 Aug 1992
- Preparation of inoculum: centrifugation (10 min at 1100 g), discard supernatant, resuspend in mineral medium, centrifugation (as above), resuspension of sludge (4.6 g dry weight/l), keep aerated
- Initial ceII concentration: 23 mg/I - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 95
- Sampling time:
- 28 d
- Details on results:
- Kinetic of test substance (in %):
= 5 after 7 day(s)
= 71 after 14 day(s)
= 93 after 21 day(s)
= 99 after 27 day(s)
= 95 after 28 day(s)
BOD5 / COD results
- Results with reference substance:
- Kinetic of control substance (in %):
= 98 after 14 day(s)
= 100 after 28 day(s)
Applicant's summary and conclusion
- Interpretation of results:
- readily biodegradable
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