3-methoxyacetophenone

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is form peer reviwed jornal

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Minimum inhibitory concentration was measure for microorganism by using by two-fold dilution method.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: Bacillus subtilis NCIM 2718, Salmonella typhi NCIM2501

Details on inoculum:

Details on test organisms
- Laboratory culture: Yes from National Chemical Laboratory, Pune, India.
- Method of cultivation:
- Preparation of inoculum for exposure: Bacteria were cultured in tryptic soy broth medium until the growth reached mid-logarithmic phase. This broth was centrifuged and washed twice with phosphate–urea–magnesium (PUM) buffer. The washed cells were resuspended in PUM buffer to reach an OD of 1.0 at 400 nm (OD400).
- Pretreatment: No data available
- Initial biomass concentration: Aliquots of 1.0 mL of suspension

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

10 min

Details on test conditions:

- Test vessel: Test tubes
- Type (delete if not applicable): open
- Test concentrations: ranging from 0–0.2 mL in steps of 0.05 mL
- Culture medium different from test medium: Tryptic soy broth medium

Reference substance (positive control):

not specified

Duration:

10 min

Dose descriptor:

other: MIC

Effect conc.:

0.063 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Validity criteria fulfilled:

not specified

Conclusions:

The Minimum Inhibitory Concentration (MIC) of Bacillus subtilis NCIM 2718 and Salmonella typhi NCIM2501 for 3-Methoxyacetophenone (586-37-8) was determined as 0.0629 mg/l.

Executive summary:

Antibacterial activity of3-Methoxyacetophenonewas observed formicroorganismdetermined by minimum inhibitory concentration using by two-fold dilution method.

Hydrophobicity was observed for the bacteria more hydrophobic the bacterial cell, the greater is its affinity to the hydrocarbon, resulting in a transfer of cells from the aqueous suspension to the organic phase, leading to a reduction in the turbidity of the culture in the former.

Optical density (OD) was measured at concentrations from 0.0 to 0.2ml.

Bacteria were cultured in medium at mid-logarithmic phase and followed the protocol. OD was measured at 400 nm and cell-free buffer served as the blank.A plot was made between OD and different hexadecane concentrations.

 

After experiment it was determined that the Minimum Inhibitory Concentration (MIC) of Bacillus subtilis NCIM 2718 and Salmonella typhi NCIM2501 for3-Methoxyacetophenone (586-37-8)was determined as 0.0629 mg/l.

Description of key information

The Minimum Inhibitory Concentration (MIC) of Bacillus subtilis NCIM 2718 and Salmonella typhi NCIM2501 for 3-Methoxyacetophenone (586-37-8) was determined as 0.0629 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

0.063 mg/L

Additional information

From peer reviwed journal (Chem Biol Drug Des 2008; 72: 303–313) the Antibacterial activity of 3-Methoxyacetophenone was observed for microorganism determined by minimum inhibitory concentration using by two-fold dilution method.

Hydrophobicity was observed for the bacteria more hydrophobic the bacterial cell, the greater is its affinity to the hydrocarbon, resulting in a transfer of cells from the aqueous suspension to the organic phase, leading to a reduction in the turbidity of the culture in the former.

Optical density (OD) was measured at concentrations from 0.0 to 0.2ml.

Bacteria were cultured in medium at mid-logarithmic phase and followed the protocol. OD was measured at 400 nm and cell-free buffer served as the blank.A plot was made between OD and different hexadecane concentrations.

 

After experiment it was determined thatthe Minimum Inhibitory Concentration (MIC) of Bacillus subtilis NCIM 2718 and Salmonella typhi NCIM2501 for3-Methoxyacetophenone (586-37-8)was determined as 0.0629 mg/l.

Other supporting study from same experimental result for different species i.e Staphylococcus aureus NCIM5021, Enterobacte aerogenes NCIM5139 and Proteus vulgaris NCIM2813 showed Minimum Inhibitory Concentration (MIC) as 0.0249 mg/l and 0.0011 mg/l respectively.

Overall experimental studies from peer reviwed articles indicate that the test substance 3-Methoxyacetophenone (586-37-8) showed minimum inhibitory effects in various micro-organisms in range 0.0011 - 0.0629 mg/l.