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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1973
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
no
Specific details on test material used for the study:
FAT 36038/A
Analytical monitoring:
no
Vehicle:
yes
Test organisms (species):
other: Rainbow trout
Details on test organisms:
Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Further standard conditions:
- Basins have been cleaned with RBS-35 solution (FLUKA 83462) and rinsed with clean water.
- Testing water should be kept at a pH between 6 and 8.5. In case of deviations, pH needs to be adjusted with NaOH or HCl solution.
- Acclimatization period: minimum 12 days at 10-13 °C for trout.
- Feeding periods: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany)
- Day-night cycles: 12/12h
- Termination: For termination of the experiment MS-222 (Sandoz) should be used at 1% (Methacain).
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
Not available
Test temperature:
15 °C
pH:
8.1-8.4
Dissolved oxygen:
None
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations: 100, 200 and 1000 mg/L
Details on test conditions:
None
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
other: TL0
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
other: TL50
Effect conc.:
450 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
other: TL100
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
None
Results with reference substance (positive control):
No reference substance tested.
Reported statistics and error estimates:
None
Validity criteria fulfilled:
yes
Conclusions:
The TL50 of FAT 36038/A in this fish acute toxicity test was determined to be 450 mg/L.
Executive summary:

The acute fish toxicity test was performed according to equivalent or similar guideline as OECD 203. Rainbow trout were used in this test. Three groups, each containing 8 fish, were tested at the following concentrations: 100, 200 and 1000 mg/L as in the pre-test all fish survived at 100 mg/L.

Results:

At 100 mg/L: 8 ou of 8 survived (pH 8.2 after 48 hours eposure)

At 200 mg/L: 8 out of 8 survived (pH 8.1 after 48 hours eposure)

At 1000 mg/L: 0 out of 8 survived at 24 hours exposure time (pH 8.4 after 24 hours eposure)

In conclusion:

TL0 = 200 mg/L

TL50 = 450 mg/L

TL100 = 1000 mg/L.

The TL50 of FAT 36038/A in this fish acute toxicity test was determined to be 450 mg/L.

Description of key information

The LC50 of FAT 36038/A in an acute fish toxicity test was determined to be 450 mg/L. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
450 mg/L

Additional information

In a key acute fish toxicity test performed following a guideline equivalent or similar to OECD Guideline 203, Rainbow trouts were exposed to FAT 36038/A for 48-hour to determine the LC values. The TL50 in the acute fish toxicity test was determined to be greater than 450 mg/L after 48 hours exposure time. In two other supporting studies, Rainbow trouts were exposed to FAT 36038 for 48 hours to determine the acute toxicity following a guideline equivalent or similar to OECD test guideline 203. The 48-hour TL50 of FAT 36038 in these fish acute toxicity tests was determined to be >1000 mg/L. Based on the data from these studies the 48-hour TL50 of FAT 36038 to fish can be considered to be 450 mg/L.