1-hydroxy-4-[[4-[(methylsulphonyl)oxy]phenyl]amino]anthraquinone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1973

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

no

Specific details on test material used for the study:

FAT 36038/A

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

other: Rainbow trout

Details on test organisms:

Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Further standard conditions:
- Basins have been cleaned with RBS-35 solution (FLUKA 83462) and rinsed with clean water.
- Testing water should be kept at a pH between 6 and 8.5. In case of deviations, pH needs to be adjusted with NaOH or HCl solution.
- Acclimatization period: minimum 12 days at 10-13 °C for trout.
- Feeding periods: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany)
- Day-night cycles: 12/12h
- Termination: For termination of the experiment MS-222 (Sandoz) should be used at 1% (Methacain).

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

Not available

Test temperature:

15 °C

pH:

8.1-8.4

Dissolved oxygen:

None

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 100, 200 and 1000 mg/L

Details on test conditions:

None

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

other: TL0

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

other: TL50

Effect conc.:

450 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

other: TL100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

None

Results with reference substance (positive control):

No reference substance tested.

Reported statistics and error estimates:

None

Validity criteria fulfilled:

yes

Conclusions:

The TL50 of FAT 36038/A in this fish acute toxicity test was determined to be 450 mg/L.

Executive summary:

The acute fish toxicity test was performed according to equivalent or similar guideline as OECD 203. Rainbow trout were used in this test. Three groups, each containing 8 fish, were tested at the following concentrations: 100, 200 and 1000 mg/L as in the pre-test all fish survived at 100 mg/L.

Results:

At 100 mg/L: 8 ou of 8 survived (pH 8.2 after 48 hours eposure)

At 200 mg/L: 8 out of 8 survived (pH 8.1 after 48 hours eposure)

At 1000 mg/L: 0 out of 8 survived at 24 hours exposure time (pH 8.4 after 24 hours eposure)

In conclusion:

TL0 = 200 mg/L

TL50 = 450 mg/L

TL100 = 1000 mg/L.

The TL50 of FAT 36038/A in this fish acute toxicity test was determined to be 450 mg/L.

Description of key information

The LC50 of FAT 36038/A in an acute fish toxicity test was determined to be 450 mg/L. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

450 mg/L

Additional information

In a key acute fish toxicity test performed following a guideline equivalent or similar to OECD Guideline 203, Rainbow trouts were exposed to FAT 36038/A for 48-hour to determine the LC values. The TL50 in the acute fish toxicity test was determined to be greater than 450 mg/L after 48 hours exposure time. In two other supporting studies, Rainbow trouts were exposed to FAT 36038 for 48 hours to determine the acute toxicity following a guideline equivalent or similar to OECD test guideline 203. The 48-hour TL50 of FAT 36038 in these fish acute toxicity tests was determined to be >1000 mg/L. Based on the data from these studies the 48-hour TL50 of FAT 36038 to fish can be considered to be 450 mg/L.