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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
no guideline available
Principles of method if other than guideline:
Short term toxicity to fish was studied for 96 hrs using test concentrations of 0, 0.1, 1.0, 10,100 and 1000 mg/l.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Lepomis macrochirus
Details on test organisms:
TEST ORGANISM
- Common name: Bluegill sunfish (Leponis Macrochirus)
- Source: Nebraska fish farm
- Length at study initiation (length definition, mean, range and SD): 67±12 mm
- Weight at study initiation (mean and range, SD): 4.9±1.7 g
- Food type: fed a dry pelleted ration ad libitum each day.

ACCLIMATION
- Acclimation period: acclimatized in holding tanks least 1week prior to exposure
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
35 mg/l CaCO3
Test temperature:
18-20°C
pH:
7.1
Nominal and measured concentrations:
Nominal:0, 0.1, 1.0, 10, 100 and 1000 mg/l
Details on test conditions:
Test vessel: 25 L pickle jars
No. of organisms per vessel: 10 fish / concentration

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality
Key result
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Validity criteria fulfilled:
not specified
Conclusions:
The LC0 value for test material for short term toxicity to fish was determined to be 1000 mg/l for 96 hrs.
Executive summary:

Acute toxicity range finding test was conducted with bluegill (Leponis Macrochirus) sunfish with test material. Ten fish per concentration were tested in 25-L pickle jars using 20L of water. Test concentrations were 0, 0.1, 1.0, 10, 100 and 1000 mg/l for sodium p-sulfophenyl methallyl ether. The test chemical was introduced as aqueous solutions without solvents prior to introducing the fish. Mortality, behavior, dissolved oxygen, pH and temperature were recorded daily for 96h.After the experiment, the LC0 value for test material for short term toxicity to fish was determined to be 1000 mg/l for 96 hrs. Based on the value, the test material was considered to be non-toxic to aquatic organism and can be considered to be not classified as per the CLP regulation.

Description of key information

Short term toxicity to fish:

Acute toxicity range finding test was conducted with bluegill (Leponis Macrochirus) sunfish with test material. Ten fish per concentration were tested in 25-L pickle jars using 20L of water. Test concentrations were 0, 0.1, 1.0, 10, 100 and 1000 mg/l for sodium p-sulfophenyl methallyl ether. The test chemical was introduced as aqueous solutions without solvents prior to introducing the fish. Mortality, behavior, dissolved oxygen, pH and temperature were recorded daily for 96h.After the experiment, the LC0 value for test material for short term toxicity to fish was determined to be 1000 mg/l for 96 hrs. Based on the value, the test material was considered to be non-toxic to aquatic organism and can be considered to be not classified as per the CLP regulation.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1 000 mg/L

Additional information

Short term toxicity to fish:

Short term toxicity of test material on fish was described in a peer reviewed journal for two differenty fish as follows:

For the fish bluegill (Leponis Macrochirus) , acute toxicity range finding test was conducted with bluegill (Leponis Macrochirus) sunfish with test material. Ten fish per concentration were tested in 25-L pickle jars using 20L of water. Test concentrations were 0, 0.1, 1.0, 10, 100 and 1000 mg/l for sodium p-sulfophenyl methallyl ether. The test chemical was introduced as aqueous solutions without solvents prior to introducing the fish. Mortality, behavior, dissolved oxygen, pH and temperature were recorded daily for 96h.After the experiment, the LC0 value for test material for short term toxicity to fish was determined to be 1000 mg/l for 96 hrs. Based on the value, the test material was considered to be non-toxic to aquatic organism and can be considered to be not classified as per the CLP regulation.

For , another fish rainbow trout acute toxicity range finding test was conducted with rainbow trout with test material. Ten fish per concentration were tested in 25-L pickle jars using 20L of water. Test concentrations were 0, 0.1, 1.0, 10, 100 and 1000 mg/l for test material. The test chemical was introduced as aqueous solutions without solvents prior to introducing the fish. Mortality, behavior, dissolved oxygen, pH and temperature were recorded daily for 96h.After the experiment, the LC0 value for test material for short term toxicity to fish was determined to be 1000 mg/l for 96 hrs.

Based on these values, the test material was considered to be non-toxic to aquatic organism and can be considered to be not classified as per the CLP regulation.