Bicyclo[3.1.1]hept-2-ene, 2,6,6-trimethyl-, phosphosulfurized

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 23 February 2016 to 25 February 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

-Diet deviated and temperature was only measured during the test period

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 23

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no data

Analytical monitoring:

no

Details on sampling:

no data

Vehicle:

no

Details on test solutions:

no data

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: E201 Daphnia magna
- Source: CEMAS (CEM Analytical Services Ltd).
- Age at study initiation (mean): = 24 hours.
- Feeding during test: The Daphnia were not fed during the test.
- Food type: A suspension of Chlorella vulgaris.
- Frequency: Each working day

CULTURE CONDITIONS
Conditions: Static
Temperature: 20 ± 2°C
Dissolved oxygen: >60% ASV
Photoperiod: 16 hours light and 8 hours dark

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

± 1 hour

Hardness:

No data

Test temperature:

The temperature was in the range of 20.3 – 21.2 °C (See attached Material)

pH:

The pH was in the range of 6.66 - 6.82

Dissolved oxygen:

The dissolved oxygen content was in the range of 6.36-6.72mg/l (See attached Material)

Salinity:

The conductivity was recorded as 0.59

Nominal and measured concentrations:

Nominal concentrations of 0 and 100mg/l with four replicates

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass containers
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume:40ml
- Aeration: limited
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 reconstituted Daphnia water
- Conductivity: 0.59

OTHER TEST CONDITIONS
- Photoperiod: 16 Hours Light, 8 Hours Dark Cycle

TEST CONCENTRATIONS
- Test concentrations: Nominal: 0, 100mg/l

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Not calculable

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Not calculable

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Not calculable

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Not calculable

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Not calculable

Duration:

24 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Not calculable

Details on results:

- Behavioural abnormalities: none
- Mortality of control: none
- Other biological observations: One daphnia trapped in the meniscus of the 100mg/l concentration. However it was still mobile.
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The dissolved concentrations were likely to be lower than stated as EXP1506833 had a 'not known' solubility (estimated to be very low) in water.

Results with reference substance (positive control):

- Results with reference substance valid
- EC50: 0.6-2.1 mg/l
- Other: done with potassium dichromate

See attached background material.

Validity criteria fulfilled:

yes

Conclusions:

The test material showed a relatively low acute toxicity to the selected test species Daphnia Magna with the EC50 being greater than 100 mg/L.

Executive summary:

The short-term toxicity of the test item to the freshwater invertebrate species Daphnia Magna was examined in accordance to the OECD guideline for the testing of chemicals 202. A water accommodated faction was prepared according to OECD guideline 23 due to the poor solubility of the test item.

A 48 hours toxicity test was performed on the fresh water invertebrate species Daphnia magna acquired from standardized laboratory cultures. The concentrations were nominal during the experiment and the EC50 value was determined by observation of mobility. The tests were conducted under controlled temperature conditions at 20.3 – 21.2 °C with a circadiac light:dark cycle. Dosing was performed under static conditions. The test substance showed low toxicity to the selected species with both the 24 and 48 hour EC50 being >100 mg/L.

Description of key information

The 48 hour EC50 of the registered substance was discovered to be greater than 100 mg/L for freshwater invertebrates species Daphnia magna. As 100 mg/L was the highest concentration tested it is proposed to adopt the conservative approach and use this concentration for the purpose of the classification. The registered substance is not considered toxic to invertebrate based on the results of this study.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The acute toxicity of the registered substance to freshwater invertebrate was determined according to OECD guideline 202 (Daphnia sp. Acute Immobilisation Test). The species used for the study was Daphnia magna (water flea) which was tested under static conditions for 48 hours. The test was carried out under controlled conditions with a temperature of 20.3 -21.2 °C with a light: dark cycle of 16:8 hours. Five organisms were kept in each vessel with four vessels (replicates) used per concentration tested. The concentrations were nominal and the EC50 determined by observation of mobility. The registered substance showed relatively low toxicity to the tested species, with both the 24 and 48 hour EC50 being greater than the highest concentration tested (100 mg/L).