Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-885-0 | CAS number: 100-74-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Justification for type of information:
- acute toxicity study performed according to OECD guideline 401, considered reliable in OECD SIDS document, compliant to GLP.
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute oral toxicity
- Author:
- Japanese government
- Year:
- 2 005
- Bibliographic source:
- OECD SIDS Initial Assessment Report for SIAM21 and OECD Agreed Conclusions
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 4-ethylmorpholine
- EC Number:
- 202-885-0
- EC Name:
- 4-ethylmorpholine
- Cas Number:
- 100-74-3
- Molecular formula:
- C6H13NO
- IUPAC Name:
- 4-ethylmorpholine
- Test material form:
- liquid
- Details on test material:
- - Alternative names of reference substance: Jeffcat NEM; N-ethylmorpholine; 4-ethylmorpholin; Morpholine, 4-ethyl-
- Molecular formula: C6H13NO
- Molecular weight: 115.17
- SMILES notation: CCN1CCOCC1
- InChl: InChI=1/C6H13NO/c1-2-7-3-5-8-6-4-7/h2-6H2,1H3
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 2901P0 (obtained from Nippon Nyukazai Co., Ltd. (Tokyo, Japan))
- Expiration date of the lot/batch: no data
- Purity test date: no data
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: stable for 8 days as diluted solution in refrigerator
- Stability under test conditions: stable for 8 days as diluted solution in refrigerator
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Crj:CD(SD)IGS rats bought from Charles River Japan, Inc.
- Age at study initiation: 4 week old
- Weight at study initiation: no data
- Fasting period before study:no data
- Housing:no data
- Diet (e.g. ad libitum):no data
- Water (e.g. ad libitum):no data
- Acclimation period: for 8 days before use
ENVIRONMENTAL CONDITIONS
- Temperature (°C):no data
- Humidity (%):no data
- Air changes (per hr):no data
- Photoperiod (hrs dark / hrs light): no data
IN-LIFE DATES: no data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Remarks:
- water for injection
- Details on oral exposure:
- VEHICLE
- no data
MAXIMUM DOSE VOLUME APPLIED:no data
DOSAGE PREPARATION (if unusual): On the previous day of the administration, the test solution was prepared and diluted to dosing concentrations by injection solvent. They were kept in a refrigerator. The diluted solution was also confirmed to be stable for 8 days
CLASS METHOD (if applicable)
no data - Doses:
- 500, 1000, 1500, 2000 mg/kg bw
- No. of animals per sex per dose:
- 40 animals in total
5 males and 5 females per group - Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:no data
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- Mean body weights and their standard deviations were calculated for every group
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 1 500 - < 2 000 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Males: mortality occurred at 1500 mg/kg bw and higher from 1 hour after treatment until day 2 of the observation period. The LD50 for NEM was estimated to be between 1500 and 2000 mg/kg bw.
- 500 mg/kg bw : 0/5
- 1000 mg/kg bw : 0/5
- 1500 mg/kg bw : 1/5
- 2000 mg/kg bw : 3/5
Females: mortality occurred at 1000 and 2000 mg/kg bw from 1 to 3 hours after treatment. The LD50 for NEM was estimated to be approx. 2000 mg/kg bw.
- 500 mg/kg bw : 0/5
- 1000 mg/kg bw : 1/5
- 1500 mg/kg bw : 0/5
- 2000 mg/kg bw : 2/5 - Clinical signs:
- Most males and females given 1000 mg/kg b.w. and higher showed tonic and/or clonic convulsions just after administration and then showed decrease in locomotor activity. These clinical signs disappeared by day 2 of observation, except for decrease of amount of stool at 1500 or 2000 mg/kg b.w. No abnormal signs were observed after day 3.
- Body weight:
- Body weight gain of both sexes was suppressed at 1500 mg/kg b.w. and higher until the day 4 of observation.
- Gross pathology:
- Dead animal: edema and red areas in the glandular stomach were found
survival animals: no abnormalities were found in the surviving animals - Other findings:
- no data
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The LD50 values for NEM are considered to be between 1500 and 2000 mg/kg bw for males and approx 2000 mg/kg bw for females. Therefore the test substance NEM is considered to be classified as category 4 according to CLP classification.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.