Ethanol, 2,2'-iminobis-, N-(C13-15-branched and linear alkyl) derivs.

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 Apr - 04 Oct 2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

(July 1992)

Deviations:

yes

Remarks:

1) test substance concentration was not measured 2) mean length of fish (6.2 cm) was above the range specified in the TG (6.0 cm) 3) loading rate (1.08 g/L) slightly exceeded the limit specified in the TG (1 g/L)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

- Stock solution: two 10 mg/L test media batches were prepared by direct addition of Atmer 163 to dilution water and stirring for ca. 4 h
- Test solutions: one batch was used directly for the 1 mg/L test medium, the second batch was diluted with water to prepare the remaining test media

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Source: Brow Well Fisheries Limited, Skipton, North Yorkshire
- Length at study initiation: 6.2 cm (5.2 - 6.9 cm)
- Weight at study initiation: 2.1566 g (1.448 - 2.9107 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: ≥ 12 days
- Type and amount of food: a proprietary food (Trouw 00), quantities depening on the size of the fish >= 24 h prior to test initiation feeding was stopped
- Health during acclimation: cumulative mortality was < 5% over a seven day period preceding the test
- Density: appropriate to fish size
- Water in tank was replaced continuously
- Photoperiod: 16 h light / 8 h dark

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

67 - 78 as CaCO3 mg/L

Test temperature:

13.0 - 13.9 °C

pH:

7.2 - 8.2

Dissolved oxygen:

86 - 106 % air saturation

Salinity:

s

Nominal and measured concentrations:

nominal: control, 0.063, 0.125, 0.25, 0.5 and 1 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria
- Type: closed; vessels were covered with perspex lids
- Material, size: glass, 15 L
- Renewal rate of test solution (semi-static conditions): renewal at 24 h intervals
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: ca. 1.08 g/L (7 fish each 2.157 g in 14 L test medium)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: mains dechlorinated water was used for the holding of stocks and for the toxicity tests
- Residual chlorine: 0.01 - 0.07 mg/L
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark


EFFECT PARAMETERS MEASURED:
- Four categories: no effects, mild toxic effects, severe toxic effects and death
- Intervals of observations: 24 h intervals (1, 24, 48, 72 and 96 h)


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.704 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

0.515 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.355 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: Behavioural abnormalities (e.g. swimming abnormally, increase cough frequency) were observed at a concentration of 0.5 mg/L after 24 h and at a concentration of 0.25 mg/L at the end of the test (96 h).
- Mortality of control: 0%

Reported statistics and error estimates:

Probit analysis after log10 transformation of the nominal concentrations.

Sublethal observations / clinical signs:

Table 1: Toxic effects exhibited in the definitive test by O. mykiss exposed to Atmer 163

Nominal concentration [mg/L]	No. Of Fish	1 h	24 h	48 h	72 h	96 h
Control	7	0	0	0	0	0
0.063	7	0	0	0	0	0
0.125	7	0	0	0	0	0
0.25	7	0	0	0	0	0
0.5	7	0	0	0	3	7
1.0	7	0	1	7	7	7

Description of key information

LC50 (96 h): 0.355 mg/L (nominal) for Oncorhynchus mykiss (OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.355 mg/L

Additional information

The acute toxicity of Atmer 163 (CAS No. 97925-95-6) to Oncorhynchus mykiss was investigated in accordance to OECD 203 under GLP conditions (Mattock, 2001). In a semi-static test system fish were exposed to nominal concentrations of 0.063, 0.125, 0.25, 0.5 and 1 mg/L. No analytical monitoring of test substance concentrations was performed. Mortality was observed at the two highest test substance concentrations and a LC50 (96 h) of 0.355 mg/L was calculated.