Disodium [2-hydroxy-3-[(2-hydroxy-1-naphthyl)azo]-5-nitrobenzene-1-sulphonato(3-)][1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)]chromate(2-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 November 1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Identification: LANACRON GRAU S-B ROH TROCKEN SFO (FAT 20'013/C)
Description: Grey solid
Batch Number: 220
Purity / Formulation: 53.15%
Stability of Test Article: Stable under specified storage conditions; expiration date: DEC-1999
Storage Conditions: In the original container at room temperature (approx. 20 °C), away from direct sunlight.
Safety Precautions: Gloves, goggles and face mask were obligatory to ensure the health and safety of the personnel.

Analytical monitoring:

not specified

Vehicle:

yes

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

21 - 22 °C

pH:

7.5 - 8.0

Dissolved oxygen:

98%

Nominal and measured concentrations:

0.1, 1, 10 and 100 mg/L

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No mortality was observed at any concentration.

Validity criteria fulfilled:

no

Conclusions:

The 48 hour EC50 and NOEC values were determined to >100 mg/L and 100 mg/L, respectively.

Executive summary:

A study was performed to assess the acute toxicity of the test item to Daphnia magna. This study was conducted in accordance with equivalent or similar guideline to OECD TG 202. Test organism were exposed to an aqueous solution of the test item at concentrations of 0.1, 1.0, 10.0 and 100 mg/L for 48 hours at a temperature of 20 °C to 22 °C under static test conditions. The number of immobilized daphnia were recorded after 24 and 48 hours. Analysis of the 100 mg/L test preparations at 0 and 48 hours showed measured test concentrations to be near nominal and so the results are based on nominal test concentrations. There was no significant immobilization in 20 daphnids exposed to a maximum test concentration of 100 mg/L for a period of 48 hours. Hence, the 48 hour EC50 and NOEC values were determined to >100 mg/L and 100 mg/L, respectively.

Description of key information

A study was performed to assess the acute toxicity of the test item to Daphnia magna. This study was conducted in accordance with equivalent or similar guideline to OECD TG 202. Test organism were exposed to an aqueous solution of the test item at concentrations of 0.1, 1.0, 10.0 and 100 mg/L for 48 hours at a temperature of 20 °C to 22 °C under static test conditions. The number of immobilized daphnia were recorded after 24 and 48 hours. Analysis of the 100 mg/L test preparations at 0 and 48 hours showed measured test concentrations to be near nominal and so the results are based on nominal test concentrations. There was no significant immobilization in 20 daphnids exposed to a maximum test concentration of 100 mg/L for a period of 48 hours. Hence, the 48 hour EC50 and NOEC values were determined to >100 mg/L and 100 mg/L, respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L