N,N,N-trimethylanilinium chloride

Endpoint summary

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio.The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 4 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item at 100 mg/l nominal test concentrations, LC50 was determine to be >100 mg/l . No mortality was observed . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Short term toxicity of aquatic invertebrate:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The stock solution 100.0 mg/l was prepared by dissolving white powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water.concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 44.0 mg/L(CI - 29.8 - 65.0 mg/l) on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The stock solution 250.0 mg/l was prepared by dissolving white powder in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Nominal50.0 , 75.0, 122.0, 170.0, 250.0 mg/lconcentration were used.With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (EC50) for the test substance , in algae was determined to be 152.1 mg/L (CI 111.2 - 208.0 mg/l) on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as per the CLP classification criteria.

Toxicity to microorganism:

The effect of test material was evaluated on microorganisms ,Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.The effect concentration  EC50 of test material on  P. phosphoreum was evaluated to be 0.22 mg/l.In another study , the test chemical was used to evaluate minimum inhibition concentration on the test organism Bacillus subtilis after inoculation of test substance , the effect concentration was observed to be 500 mg/l. The effect of test material on bioluminence of bacterial P.phosphorium and growth inhibition of Bacillus subtilis was observed to be 0.22 mg/l and 500 mg/l respectively.

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio.The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 4 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item at 100 mg/l nominal test concentrations, LC50 was determine to be >100 mg/l . No mortality was observed . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Short term toxicity of aquatic invertebrate:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The stock solution 100.0 mg/l was prepared by dissolving white powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water.concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 44.0 mg/L(CI - 29.8 - 65.0 mg/l) on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The stock solution 250.0 mg/l was prepared by dissolving white powder in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Nominal50.0 , 75.0, 122.0, 170.0, 250.0 mg/lconcentration were used.With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (EC50) for the test substance , in algae was determined to be 152.1 mg/L (CI 111.2 - 208.0 mg/l) on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as per the CLP classification criteria.

Toxicity to microorganism:

The effect of test material was evaluated on microorganisms ,Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.The effect concentration  EC50 of test material on  P. phosphoreum was evaluated to be 0.22 mg/l.In another study , the test chemical was used to evaluate minimum inhibition concentration on the test organism Bacillus subtilis after inoculation of test substance , the effect concentration was observed to be 500 mg/l. The effect of test material on bioluminence of bacterial P.phosphorium and growth inhibition of Bacillus subtilis was observed to be 0.22 mg/l and 500 mg/l respectively.