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Administrative data

Description of key information

Geranyl nitrile is likely to be non hazardous by oral, inhalation and dermal route of exposure.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records
Reference
Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal
Qualifier:
according to guideline
Guideline:
other: refer below principle
Principles of method if other than guideline:
The acute toxicity study has been performed on rats by oral route.
GLP compliance:
not specified
Test type:
other: no data
Limit test:
no
Species:
rat
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
No details available
Route of administration:
oral: unspecified
Vehicle:
not specified
Details on oral exposure:
No data
Doses:
No data
No. of animals per sex per dose:
No data
Control animals:
not specified
Details on study design:
No details available
Statistics:
No data
Sex:
not specified
Dose descriptor:
LD50
Effect level:
3 100 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50 % mortaltiy
Mortality:
50% mortality occurred
Clinical signs:
No data
Body weight:
No data
Gross pathology:
No data
Other findings:
No data
Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The LD50 value for test chemical Geranyl nitrile was found to be 3100 mg/kg when exposed to rat by oral route.
Executive summary:

The acute toxicity study has been conducted on rats by oral route for the chemical Geranyl nitrile.The LD50 value was found to be 3100 mg/kg when exposed to rat by oral route.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
3 100 mg/kg bw
Quality of whole database:
Data is Klimisch 2 and from peer-reviewed journal

Acute toxicity: via inhalation route

Link to relevant study records
Reference
Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal and study report by Commission of the European Communities
Qualifier:
according to guideline
Guideline:
other: refer below principle
Principles of method if other than guideline:
Acute inhalation toxicity of chemical Geranyl nitrile in rats.
GLP compliance:
not specified
Test type:
other: no data
Limit test:
yes
Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
No details available
Route of administration:
inhalation
Type of inhalation exposure:
nose/head only
Vehicle:
not specified
Details on inhalation exposure:
4-hrs
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
Concentrations:
5.2 mg/L
No. of animals per sex per dose:
10 male,10 female
Control animals:
no
Details on study design:
- Duration of observation period following administration: 4 days
- Frequency of observations and weighing:no data
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology
Statistics:
No data available
Preliminary study:
No data available
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 5.2 mg/L air
Based on:
test mat.
Exp. duration:
4 h
Remarks on result:
other: No mortality observed
Mortality:
No mortality were observed in treated male and female rats at 5.2 mg/L
Clinical signs:
other: During exposure aqueous discharge from noses and salivation, slight discharge from eyes, tips of noses, ears and limbs reddened. After exposure tips of noses reddened with limited loss of hair.
Body weight:
No data
Gross pathology:
After 4 days animals without findings,Necropsy revealed no abnormalities in sacrificed animals
Other findings:
No data
Interpretation of results:
practically nontoxic
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
LC 50 was considered to be > 5.2 m/L when male and female rats were treated with 3,7-Dimethylocta-2,6-dienenitrile by Liquid aerosol exposure in Head nose inhalation system for 4 hours.
Executive summary:

In a acute inhalation toxicity study, male and female rats were treated with 3,7-Dimethylocta-2,6-dienenitrile in the concentration of 5.2 m/L by Liquid aerosol exposure in Head nose inhalation system for 4 hours. No effect on survival of treated male and female rats was observed at 5.2 m/L. During exposure aqueous discharge from noses and salivation, slight discharge from eyes, tips of noses, ears and limbs reddened. After exposure tips of noses reddened with limited loss of hair. After 4 days, no clinical sign were observed in treated rats. In addition, no gross pathological changes were observed in treated male and female rats. Therefore, LC 50 was considered to be > 5.2 m/L when male and female rats were treated with 3,7-Dimethylocta-2,6-dienenitrile by Liquid aerosol exposure in Head nose inhalation system for 4 hours.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LC50
Value:
520 mg/m³
Quality of whole database:
Data is Klimisch 2 and from peer-reviewed journal

Acute toxicity: via dermal route

Link to relevant study records
Reference
Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal
Qualifier:
according to guideline
Guideline:
other: refer below principle
Principles of method if other than guideline:
The acute toxicity study has been performed on rabbits by dermal route.
GLP compliance:
not specified
Test type:
other: no data
Limit test:
no
Species:
rabbit
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
No details available
Type of coverage:
not specified
Vehicle:
not specified
Details on dermal exposure:
no data
Duration of exposure:
no data
Doses:
4300 mg/kg bw
No. of animals per sex per dose:
no data
Control animals:
not specified
Details on study design:
no data
Statistics:
no data
Preliminary study:
no data
Sex:
not specified
Dose descriptor:
LD50
Effect level:
4 300 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50 % mortality
Mortality:
50% mortality occurred
Clinical signs:
No data
Body weight:
No data
Gross pathology:
No data
Other findings:
No data
Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
LD50 was considered to be 4300 mg/kg bw when rabbits were treated with Geranyl nitrile by dermal application.
Executive summary:

In a acute dermal toxicity study, rabbits were treated with Geranyl nitrile in the concentration of 4300 mg/kg bw by dermal application. 50 % mortality observed in 4300 mg/kg bw treated rats. Therefore, LD50 was considered to be 4300 mg/kg bw when rabbits were treated with Geranyl nitrile by dermal application.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
4 300 mg/kg bw
Quality of whole database:
Data is Klimisch 2 and from peer-reviewed journal

Additional information

Acute oral toxicity:

Based on the data available for target Geranyl nitrile (CAS no 5146-66-7) and its read across 3,7-Dimethyl-2,6-nonadienenitrile (CAS no 61792-11-8) and Citronellyl Nitrile (CAS no 51566-62-2) are summarized below

In a study given in monographs by Wohlet al(1976), acute oral toxicity was evaluated in rat by using Geranyl nitrile in the concentration of 3100 mg/kg bw orally. 50 % mortality observed in 3100 mg/kg bw treated rats. Therefore, LD50 was considered to be 3100 mg/kg bw when rat were treated with Geranyl nitrile orally.

Based on the prediction done by using QSAR Toolbox 3.4 (2016), acute oral toxicity was estimated in Wistar male and female rats by using geranyl nitrile orally. 50 % mortality observed in 3472.5 mg/kg bw treated rats. Therefore, estimated LD50 was considered to be 3472.5 mg/kg bw when Wistar male and female rats were treated with geranyl nitrile orally

In a study given in monographs by Morenoet al(1992) for read across, acute oral toxicity was evaluated rat by using 3,7-Dimethyl-2,6-nonadienenitrile in the concentration of 2600 mg/kg bw orally. 50 % mortality observed in 2600 mg/kg bw treated rats. Therefore, LD50 was considered to be 2600 mg/kg bw when rat were treated with 3,7-Dimethyl-2,6-nonadienenitrile orally.  

In a study given in monographs by Morenoet al(1979 for read across), acute oral toxicity was evaluated rat by using Citronellyl Nitrile in the concentration of 5300 mg/kg bw orally. 50 % mortality observed in 5300 mg/kg bw treated rats. Therefore, LD50 was considered to be 5300 mg/kg bw (4200—6700) when rat were treated with Citronellyl Nitrile orally.   

 

Thus, based on weight of evidence for target Geranyl nitrile (CAS no 5146-66-7) and its read across 3,7-Dimethyl-2,6-nonadienenitrile (CAS no 61792-11-8) and Citronellyl Nitrile (CAS no 51566-62-2) is likely to be non hazardous by oral route of exposure.

Acute inhalation toxicity:

Based on the data available for target Geranyl nitrile (CAS no 5146-66-7) are summarized below

In a study given by Brainet al(2007) and Commission of European Communities (2007), acute inhalation toxicity was evaluated in male and female rats by using 3,7-Dimethylocta-2,6-dienenitrile in the concentration of 5.2 m/L by Liquid aerosol exposure in Head nose inhalation system for 4 hours. No effect on survival of treated male and female rats was observed at 5.2 m/L. During exposure aqueous discharge from noses and salivation, slight discharge from eyes, tips of noses, ears and limbs reddened. After exposure tips of noses reddened with limited loss of hair. After 4 days, no clinical sign were observed in treated rats. In addition, no gross pathological changes were observed in treated male and female rats. Therefore, LC 50 was considered to be > 5.2 m/L when male and female rats were treated with 3,7-Dimethylocta-2,6-dienenitrile by Liquid aerosol exposure in Head nose inhalation system for 4 hours.

Based on the prediction done by using QSAR Toolbox 3.4 (2016), acute inhalation toxicity was estimated in Wistar male and female rats by using geranyl nitrile in the concentration of 3.4 m/L by aerosol exposure in Head nose inhalation system for 4 hours. 50 % effect on survival of treated male and female rats was observed at 3.4 m/L. Therefore, LC 50 was considered to be 3.4 m/L when Wistar male and female rats were treated with geranyl nitrile by Liquid aerosol exposure in Head nose inhalation system for 4 hours.

Thus, based on weight of evidence for target Geranyl nitrile (CAS no 5146-66-7) is likely to be non hazardous by inhalation route of exposure.

Acute dermal toxicity:

Based on the data available for target Geranyl nitrile (CAS no 5146-66-7) and its read across 3,7-Dimethyl-2,6-nonadienenitrile (CAS no 61792-11-8), 3-Methyl-2(3)-Nonenenitrile (CAS no 53153-66-5) and Citronellyl Nitrile (CAS no 51566-62-2) are summarized below

In a study given in monographs by Wohlet al(1976), acute dermal toxicity was evaluated in rabbits by using Geranyl nitrile in the concentration of 4300 mg/kg bw by dermal application. 50 % mortality observed in 4300 mg/kg bw treated rats. Therefore, LD50 was considered to be 4300 mg/kg bw when rabbits were treated with Geranyl nitrile by dermal application.

Based on the prediction done by using QSAR Toolbox 3.4 (2016), acute dermal toxicity was estimated in rabbits by using geranyl nitrile by dermal route. 50 % mortality observed in 2698.4 mg/kg bw treated rabbits. Therefore, estimated LD50 was considered to be 2698.4 mg/kg bw when rabbits were treated with geranyl nitrile by dermal route.

In a study given in monographs by Morenoet al(1992) for read across, acute dermal toxicity was evaluated in 10 rabbits by using 3,7-Dimethyl-2,6-nonadienenitrile in the concentration of 5000 mg/kg bw by dermal application. No mortality (0/10) was observed in 5000 mg/kg bw treated rabbits. Therefore, LD50 was considered to be > 5000 mg/kg bw when rabbits were treated with 3,7-Dimethyl-2,6-nonadienenitrile by dermal application.

In a study given in monographs by Morenoet al(1982) for read across, acute dermal toxicity was evaluated in rabbits by using 3-Methyl-2(3)-Nonenenitrile in the concentration of 5000 mg/kg bw by dermal application. No mortality was observed in 5000 mg/kg bw treated rabbits. Therefore, LD50 was considered to be > 5000 mg/kg bw when rabbits were treated with 2-Nonenenitrile, 3-methyl- by dermal application.  

In a study given in monographs by Morenoet al(1979) for read across, acute dermal toxicity was evaluated in rabbits by using Citronellyl Nitrile in the concentration of 5000 mg/kg bw dermal application. No mortality observed in 5000 mg/kg bw treated rabbits. Therefore, LD50 was considered to be > 5000 mg/kg bw when rabbits were treated with Citronellyl Nitrile by dermal application. 

Thus, based on weight of evidence for target Geranyl nitrile (CAS no 5146-66-7) and its read across 3,7-Dimethyl-2,6-nonadienenitrile (CAS no 61792-11-8), 3-Methyl-2(3)-Nonenenitrile (CAS no 53153-66-5) and Citronellyl Nitrile (CAS no 51566-62-2) is likely to be non hazardous by dermal route of exposure.

Justification for selection of acute toxicity – oral endpoint

LD50 was considered to be 3100 mg/kg bw when rat were treated with Geranyl nitrile orally.

Justification for selection of acute toxicity – inhalation endpoint

LC 50 was considered to be > 5.2 m/L when male and female rats were treated with 3,7-Dimethylocta-2,6-dienenitrile by Liquid aerosol exposure in Head nose inhalation system for 4 hours.

Justification for selection of acute toxicity – dermal endpoint

LD50 was considered to be 4300 mg/kg bw when rabbits were treated with Geranyl nitrile by dermal application.

Justification for classification or non-classification

Based on weight of evidence for target Geranyl nitrile (CAS no 5146-66-7) and its read across 3,7-Dimethyl-2,6-nonadienenitrile (CAS no 61792-11-8), 3-Methyl-2(3)-Nonenenitrile (CAS no 53153-66-5) and Citronellyl Nitrile (CAS no 51566-62-2) is likely to be non hazardous by oral, inhalation and dermal route of exposure.