3,7-dimethylocta-2,6-dienenitrile

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal and study report by Commission of the European Communities

Data source

Reference

Reference 1

Reference Type:

publication

Title:

In-vitro human skin penetration of the fragrance material geranyl nitrile

Author:

K.R. Brain a, D.M. Green a, J. Lalko b, A.M. Api b

Year:

2007

Bibliographic source:

Toxicology in Vitro 21 (2007) 133–138

Reference 2

Reference Type:

review article or handbook

Title:

Commission of the European Communities DG XI

Author:

Prepared by-Germany

Year:

2007

Bibliographic source:

CLASSIFICATION AND LABELLING OF DANGEROUS SUBSTANCES Recommended form to be used for the proposed classification and labelling of a dangerous substance under Directive 67/548/EEC, January 2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: refer below principle

Principles of method if other than guideline:

Acute inhalation toxicity of chemical Geranyl nitrile in rats.

GLP compliance:

not specified

Test type:

other: no data

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,7-dimethylocta-2,6-dienenitrile

EC Number:

225-918-0

EC Name:

3,7-dimethylocta-2,6-dienenitrile

Cas Number:

5146-66-7

Molecular formula:

C10H15N

IUPAC Name:

3,7-dimethylocta-2,6-dienenitrile

Test material form:

other: Liquid

Details on test material:

- Name of test material (as cited in study report): Geranyl nitrile
- Molecular formula (if other than submission substance): C10H15N
- Molecular weight (if other than submission substance): 149.236 g/mole
- Smiles notation (if other than submission substance): N#C\C=C(\CC\C=C(\C)C)C
- InChl (if other than submission substance): 1S/C10H15N/c1-9(2)5-4-6-10(3)7-8-11/h5,7H,4,6H2,1-3H3/b10-7+
- Substance type: Organic
- Physical state: Liquid

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

No details available

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

nose/head only

Vehicle:

not specified

Details on inhalation exposure:

4-hrs

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

4 h

Concentrations:

5.2 mg/L

No. of animals per sex per dose:

10 male,10 female

Control animals:

no

Details on study design:

- Duration of observation period following administration: 4 days
- Frequency of observations and weighing:no data
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology

Statistics:

No data available

Results and discussion

Preliminary study:

No data available

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 5.2 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Remarks on result:

other: No mortality observed

Mortality:

No mortality were observed in treated male and female rats at 5.2 mg/L

Clinical signs:

other: During exposure aqueous discharge from noses and salivation, slight discharge from eyes, tips of noses, ears and limbs reddened. After exposure tips of noses reddened with limited loss of hair.

Body weight:

No data

Gross pathology:

After 4 days animals without findings,Necropsy revealed no abnormalities in sacrificed animals

Other findings:

No data

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

LC 50 was considered to be > 5.2 m/L when male and female rats were treated with 3,7-Dimethylocta-2,6-dienenitrile by Liquid aerosol exposure in Head nose inhalation system for 4 hours.

Executive summary:

In a acute inhalation toxicity study, male and female rats were treated with 3,7-Dimethylocta-2,6-dienenitrile in the concentration of 5.2 m/L by Liquid aerosol exposure in Head nose inhalation system for 4 hours. No effect on survival of treated male and female rats was observed at 5.2 m/L. During exposure aqueous discharge from noses and salivation, slight discharge from eyes, tips of noses, ears and limbs reddened. After exposure tips of noses reddened with limited loss of hair. After 4 days, no clinical sign were observed in treated rats. In addition, no gross pathological changes were observed in treated male and female rats. Therefore, LC 50 was considered to be > 5.2 m/L when male and female rats were treated with 3,7-Dimethylocta-2,6-dienenitrile by Liquid aerosol exposure in Head nose inhalation system for 4 hours.