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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No OECD guideline or GLP defined.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2009

Materials and methods

Objective of study:
other: Identification of an N-acetylcysteine conjugate in the urine after oral administration of 2,4-dichloro-1-nitrobenzene to rats
Principles of method if other than guideline:
other: Identification of an N-acetylcysteine conjugate in the urine after oral administration of 2,4-dichloro-1-nitrobenzene to rats
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1,3-dichloro-4-nitrobenzene
EC Number:
210-248-3
EC Name:
1,3-dichloro-4-nitrobenzene
Cas Number:
611-06-3
Molecular formula:
C6H3Cl2NO2
IUPAC Name:
1,3-dichloro-4-nitrobenzene
Details on test material:
no data
Radiolabelling:
yes

Test animals

Species:
rat
Strain:
Fischer 344/DuCrj
Sex:
male

Administration / exposure

Route of administration:
oral: feed
Vehicle:
other: Test substance mixed into CRF-1 powdered diet.
Duration and frequency of treatment / exposure:
rats were fed the diet containing the test substance for 2 days.
Doses / concentrations
Remarks:
Doses / Concentrations:
diet containing 1.0% test substance
No. of animals per sex per dose / concentration:
3
Control animals:
yes

Results and discussion

Toxicokinetic / pharmacokinetic studies

Details on absorption:
see: executive summary
Details on distribution in tissues:
see: executive summary
Transfer into organs
Observation:
other: see: executive summary
Details on excretion:
see: executive summary
Toxicokinetic parameters
Toxicokinetic parameters:
other: see: executive summary

Any other information on results incl. tables

see: executive summary

Applicant's summary and conclusion

Executive summary:

In an oral study in male F344/DuCrj rats, the animals received diet containing 1.0% test substance for 3 days.

The objective of this study was to identify the chemical structure of the test substance metabilites in urine. Urine from the test substance fed rats was more yellow than urine from control rats, exhibiting a broad UV-spectrum around a peak wavelength of 360 nm; the control urine did not have an absorbance. The yellow component was extracted and analyzed. The optical properties of the yellow component were the same as the N-acetylcysteine conjugate of 1,4 -dichloro-2 -nitrobenzene: 1,4 -DCNB is secreted in urine as an N-acetylcysteine conjugate. LC-MS/MS analyses of this yellow component demonstrated its chemical structure to be the N-acetylcysteine conjugate of the test substance. Nuclear overhauser effect and LC-MS/MS analyses revealed the structural isomer of this test substance metabolite as N-acetyl-S-(5 -chloro-2 -nitrophenyl)-L-cysteine.

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