Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

Based on the most sensitive parameter (mean number of cumulative live offspring

per female), the EC10 value for 1,3-bis(3-methyl-2,5-dioxo-1H-pyrrolinylmethyl)benzene and Daphnia magna were calculated to be 0.205 mg/L.
The 21 day NOEC and LOEC values for reproduction were determined to be 0.800 and > 0.800 mg/L, respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.205 mg/L

Additional information

In order to test the chronic toxicity to invertebrates of 1,3-bis(3-methyl-2,5-dioxo-1H-pyrrolinylmethyl)benzene, Daphnia magna was exposed to the test solution of five nominal concentrations (0.026, 0.064, 0.16, 0.40 and 1.0 mg/L), control and solvent control for a period of 21 days under flow-through conditions. According to OECD guideline 211 (Daphnia magna Reproduction Test) (2008), the following results were obtained:

For Offspring per female (reproduction) an EC10 of 0.205 mg/L and an EC50 of >0.800 mg/L were calculated. A LOEC of >0.800 mg/L and a NOEC of 0.800 mg/L was determined.

For Survival of parental (Mortalibitity) an EC10 of 0.342 mg/L and an EC50 of >0.800 mg/L were calculated. A LOEC of 0.800 mg/L and a NOEC of 0.334 mg/L was determined.

For Length of female an EC10 of >0.800 mg/L and an EC50 of >0.800 mg/L were calculated. A LOEC of 0.334 mg/L and a NOEC of 0.151 mg/L was determined.

The first brood of offspring was on exposure day 7.

Recoveries of the 0.026, 0.064, 0.16, 0.4 and 1.0 mg/L 1,3-bis(3-methyl-2,5-dioxo-1H-pyrrolinylmethyl)benzene samples measured on day -4 ranged from 131 to 152 % of the nominal fortified levels. Based on these results and the verification of flow rates of the diluter, the proper functioning of the diluter was illustrated four days before experimental start.