3-methylbut-2-en-1-ol

Skin irritation / corrosion

Administrative data

Endpoint:

skin corrosion: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study conducted in compliance with GLP regulations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: OECD Guideline for testing of chemicals, draft proposal for a new guideline: "In vitro Skin Corrosion tests", Draft November 1999

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.40 (In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test (TER))

Principles of method if other than guideline:

EpiDerm skin corrosivity test.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-2-en-1-ol

EC Number:

209-141-4

EC Name:

3-methylbut-2-en-1-ol

Cas Number:

556-82-1

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-2-en-1-ol

Details on test material:

- Name of test substance: 3-Methylbut-2-en-1-ol (Prenol)
- Test substance No.: 00/0274-1
- Batch No.: Ch. 00/18, Abl. Nr. 56-1706
- Physical state / appearance : Liquid / colorless - clear
- Degree of purity/content: 98.8 area %
- Homogeneity: The test substance was homogeneous by visual inspection.
- Stability: The stability under storage conditions was confirmed by analysis up to March 2002 Due to the short study period, no further reanalysis was performed. The analysis was carried out at the Analytical Department, BASF Aktiengesellschaft.
- Storage conditions: Room temperature

In vitro test system

Test system:

human skin model

Source species:

human

Cell type:

non-transformed keratinocytes

Cell source:

other: EpiDerm System

Vehicle:

water

Control samples:

yes, concurrent vehicle

Duration of treatment / exposure:

3, 60 minutes

Duration of post-treatment incubation (if applicable):

3 hours (in MTT solution)

Number of replicates:

2 tissues per test material and condition

Test system

Amount / concentration applied:

undiluted

Results and discussion

In vitro

Results

Results 1

Irritation / corrosion parameter:

% tissue viability

Run / experiment:

3 min

Value:

92

Vehicle controls validity:

valid

Negative controls validity:

valid

Positive controls validity:

valid

Results 2

Irritation / corrosion parameter:

% tissue viability

Run / experiment:

60 min

Value:

10

Vehicle controls validity:

valid

Negative controls validity:

valid

Positive controls validity:

valid

Other effects / acceptance of results:

Viability of the test substance treated tissues determined after an exposure period of 3 minutes was 92% and for the exposure period 1 hour 10% .
The test substance is not able to directly reduce MTT .

Any other information on results incl. tables

Table 1: Findings

Exposure: 3 min
Test-Article	OD570, tissue 1	OD570, tissue 2	mean OD570	viability (% of negative control)
negative control	1.947	1.734	1.841	100
test substance	1.741	1.656	1.699	92
positive control	0.359	0.404	0.383	22
Exposure: 1 h
Test-Article	OD570, tissue 1	OD570, tissue 2	mean OD570	viability (% of negative control)
negative control	1.627	1.748	1.687	100
test substance	0.164	0.178	0.171	10
positive control	0.246	0.204	0.225	13