3-methylbut-2-en-1-ol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions (only 4 Samonella typhimurium strains tested)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Remarks:

The test was conducted under GLP working conditions, but not yet certified.

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-2-en-1-ol

EC Number:

209-141-4

EC Name:

3-methylbut-2-en-1-ol

Cas Number:

556-82-1

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-2-en-1-ol

Details on test material:

- Name of test material (as cited in study report): 3-Methylbuten-2-ol-1, No.: 88/360
- Analytical purity: 99 %
- Lot/batch No.: B 2391
- Storage condition of test material: Room temperature

Method

Target gene:

For Salmonella typhimurium strains, the amino acid histidine locus is the target gene.

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

S-9 mix from Arochlor 1254 induced liver of 5 male Sprague-Dawley rats

Test concentrations with justification for top dose:

In both, the standard plate test and the preincubation test: 20; 100; 500; 2500 and 5000 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: water
- Justification for choice of solvent/vehicle: Complete solubility of the test substance in aqua dest.

Controls

Controls 1

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Remarks:

10 µg (dissolved in DMSO) was used in the presence of S-9 mix in all investigated strains

Controls 2

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)

Remarks:

5 µg (dissolved in DMSO) was used without the presence of S-9 mix in the strains TA 100 and TA 1535

Controls 3

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: 4-nitro-o-phenylendiamine

Remarks:

10 µg (dissolved in DMSO) was used without the presence of S-9 mix in strain TA 98

Controls 4

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: 9-aminoacridine chloride monohydrate

Remarks:

100 µg (dissolved in DMSO) was used without the presence of S-9 mix in strain TA 1537

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation); preincubation;

DURATION
- Preincubation period: 20 min (preincubation test)
- Exposure duration: 48 h

INDICATION FOR MUTAGENICITY: An increase in the number of revertants (his+).

DETERMINATION OF CYTOTOXICITY
- relative total growth; (reduced background growth of histidine-negative bacteria, decrease in the number of histidine-positive revertants)

Evaluation criteria:

In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results

Statistics:

The mean number of revertant colonies per plate, titer, and the standard deviations were calculated for all dose groups as well as for the positive and negative (vehicle) controls in all experiments.

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: only in TA 100 in the preincubation test with S-9 mix from about 100 µg/plate onward

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

No additional results

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Plate Incorporation test (20 - 5000 µg/plate)
Strain	Metabolic activation system	mean revertants in Controls	maximum revertant factor	dose dependency	Assessment
TA 100	no	111	1.2	no	negative
	yes	111	1.0	no	negative
TA 98	no	31	0.9	no	negative
	yes	43	1.0	no	negative
TA 1535	no	25	0.8	no	negative
	yes	23	1.2	no	negative
TA 1537	no	8	1.1	no	negative
	yes	10	1.1	no	negative

Preincubation test (20 - 5000 µg/plate)
Strain	Metabolic activation system	mean revertants in Controls	maximum revertant factor	dose dependency	Assessment
TA 100	no	118	1.0	no	negative
	yes	106	1.0	no	negative
TA 98	no	23	0.9	no	negative
	yes	29	1.2	no	negative
TA 1535	no	20	1.1	no	negative
	yes	21	1.0	no	negative
TA 1537	no	7	1.0	no	negative
	yes	5	1.7	no	negative