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EC number: 249-707-8
CAS number: 29590-42-9
Three studies have been conducted: An Ames Assay, a Chromosome
Aberration study and a Mouse Lymphoma assay.
In the Chromosome aberration study, MTDID 7819 did not induce a
statistically significant or biologically relevant increase in the
number of cells with chromosome aberrations in the absence of S9-mix at
the 3 hour and 24 hour exposure times and in the presence of S(-mix, in
the first and second cytogenetic assays. However, in the second
cytogenetic assay in the absence of S9-mix at the 48-hour exposure time,
MTDID-7819 induced a statistically significant increase in the number of
cells with chromosome aberrations at the highest, cytotoxic
concentration, both when gaps were included and excluded. Because this
increase was above the historical control data range, observed in both
duplicate cultures it was considered biologically relevant and
MTDID-7819 was considered clastogenic.
No effects of MTDID-7819 on the number of polyploid cells and cells with
endoreduplicated chromosomes were observed both in the absence and
presence of S9-mix. Therefore, it can be concluded that MTDID-7819 does
not disturb mitotic processes and cell cycle progression and does not
induce numerical chromosome aberrations under the experimental
conditions described in the report.
In the Mouse Lymphoma study, the test article was tested in the presence
and absence of exogenous metabolic activation by Aroclor induced rat
liver S-9 in the L5178Y TK+/- Mouse Lymphoma Mutagenesis Assay. Although
some of the test cultures exhibited mutant frequencies which were two
fold greater than the average mutant frequency of the corresponding
solvent control, no dose response was seen and the mutant frequencies
were within the range of experimental error (mutant frequencies similar
to those for solvent controls for the positive controls). The test
article was therefore considered negative in the assay under the test
In the Ames Salmonella/microsome assay, Compound T-2476ChR was tested
initially in a preliminary assay with S. typhimurium strain TA100 over a
wide range of concentrations, from 0.01 to 5.0 ul/plate Pinpoint
colonies (indicating toxicity) appeared at 5.0 ul/plate, so the
concentration range was lowered to 0.0005 to 0.5 ul/plate in subsequent
tests using all five strains of S. typhimurium. At 0.5 ul/plate, the
test compound was toxic with TA100, but no dose-related increase in the
number of revertants per plate was observed in either assay.
In the microbiological assays with S. cerevisiae D3, T-2476ChR was
initially tested over a wide range of concentrations, from 0.01 to 5.0%.
Toxicity was observed at all concentrations so the range was lowered to
0.00005 to 0.05% for subsequent testing. No dose-related increase in the
number of mitotic recombinants above background was observed.
The test material, T-2476ChR was negative in this assay.
The criteria for classification were not met.
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