Bis(2,3-epoxypropyl) cyclohex-4-ene-1,2-dicarboxylate

Toxicity to aquatic algae and cyanobacteria

Description of key information

Toxicity of bis(2,3-epoxypropyl)cyclohexane-1,2-dicarboxylate to Pseudokirchneriella subcapitata in an Algal Growth Inhibition Test according to OECD 201, EU method C.3. and ISO 8692.

Key value for chemical safety assessment

EC50 for freshwater algae:

56 mg/L

EC10 or NOEC for freshwater algae:

18 mg/L

Additional information

The acute Toxicity of bis(2,3-epoxypropyl)cyclohexane-1,2-dicarboxylate to the unicellular green algal species Pseudokirchneriella subcapitata was determined in a static 72 hours test. The test was conducted in accordance with OECD Guideline 201, EU Method C.3 and ISO 8692. This study encompassed 6 treatment groups (5 dose rates of the test item:100, 32, 10, 3.2 and 1 mg test item/L (nominal), corresponding to 56, 19, 5.1, 1.8 and 0.64 mg test item/L Time Weighted Average (TWA) values,and one control) with three replicates per test concentration and six replicates for the control. Under the conditions of the present study with Pseudokirchneriella subcapitata, bis(2,3-epoxypropyl) cyclohexane-1,2-dicarboxylate reduced growth rate and inhibited the yield of this fresh water algae species significantly at TWA concentrations of 1.8 mg/l and higher.

The EC50 for growth rate reduction (ErC50: 0-72h) exceeded a TWA concentration of 56 mg/l. It was expected that the ErC50 was just above this concentration.The EC50 for yield inhibition (EyC50: 0-72h) was 18 mg/l with a 95% confidence interval ranging from 11 to 30 mg/l.The NOEC for both growth rate reduction and yield inhibition was 0.64 mg/l based on TWA concentrations.