Benzenesulfonic acid, mono-C10-13-alkyl derivs., compds. with diethanolamine

Long-term toxicity to fish

Description of key information

There is no experimental data available for the test item. Alternatively, a read-across approach is applied. As lowest threshold value the following value was obtained in fish for the source substance:

NOEC = 0.23 mg LAS Na/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.23 mg/L

Additional information

No experimental data on long term toxicity to fish is available for the test item. Alternatively, experimental data for a source substance is available and a read-across approach is applied. Please refer to read-across justification document attached in IUCLID Section 13. For further supporting information please refer to respective REACH registration dossier of the source substance.

Source substance 2 (LAS NA, CAS 68411-30-3)

 

In a 196 day long-term fish life cycle test, Fathead minnow (Pimephales promelas) were exposed to Benzenesulfonic acid (C10-13-alkyl derivs., sodium salts, CAS 68411-30-3) in an open system. Nominal test concentrations were 0.34, 0.63, 1.2 and 2.7 mg/L. The 196 d NOEC, based on geometric mean measured concentration, was 0.63 mg/L; the 196 d LOEC was 1.2 mg/L. Results indicate that lethality of the test item to newly hatched fry was the most critical factor found. This toxicity study is classified as acceptable, as it is well documented and meets generally accepted scientific principles.

In a 90 day juvenile growth test, Mozambique tilapia (Tilapia mossambica) were exposed to Benzenesulfonic acid (C10-13-alkyl derivs., sodium salts, CAS 68411-30-3) in an open system. Groups of 15 fish were exposed to nominal test concentrations of 0.25, 0.38, 0.51, and 1.10 mg/L. Test solutions were renewed every 15 days. The 90-day NOEC was 0.25 mg/L, and the LOEC was 0.51 mg/L. This toxicity study is classified as acceptable, as it is well documented and meets generally accepted scientific principles.

 

In a 28 day juvenile growth test, 50 Guppy (Poecilia reticulata) were exposed to Benzenesulfonic acid (C10-13-alkyl derivs., sodium salts, CAS 68411-30-3) in a semi-static system. Nominal concentrations were not specified in the report. The only effect (98% mortality at 10 mg/L) occurred within 2 days of study initiation. Test solution was renewed three times per week. The 28-day NOEC for fish was 3.2 mg/L (nominal). The 28-day LOEC was 10 mg/L (nominal). This toxicity study is classified as acceptable, as it is well documented and meets generally accepted scientific principles.

Fertilized eggs of Rainbow trout (Salmo gairdneri) were exposed to concentrations of 0.03, 0.23, 0.35, 0.63, 0.95 and 1.9 mg/L (mean measured), for 70 days. The responses recorded included the survival of eggs, time to eyed egg stage, time to hatch, survival and final weight of sac-fry (eleutheroembryos), time and extent of swim-up (external feeding). Test solution was renewed every week. The lowest NOEC value found was 0.23 mg/L based on survival of eggs exposed from eyed stage, survival of eggs exposed from fertilization, survival of sac fry, and overall survival from fertilization to swim-up. This toxicity study is classified as acceptable, as it is well documented and meets generally accepted scientific principles.