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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

A manometric respirometry study assessing the ready biodegradability of 3,3,5-TRIMETHYLCYCLOHEXYL ACRYLATE (CAS 86178-38-3) was conducted in accordance with the OECD 301 F Test Guideline and GLP requirements.

The test item was not readily biodegradable under the conditions of the test. A mean total of 16.8% biodegradation was achieved by Day 28 and 47.5% by day 56.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable, not fulfilling specific criteria

Additional information

The study was conducted in accordance with procedures outlined in OECD Guideline 301F (1992). The ready biodegradability of 3.3.5-trimethylcyclohexyl acrylate was assessed over a 28 days period with an extended period up to 56 days.

 

As test item is known to be difficult to dissolve in water, its dispersion in the medium was improved by using an emulsifying agent (Symperonic P103) as described in ISO 10634 standard.

 

A total of 9 bioreactors were used:

 

- 2 each for test item, blank control, reference item control, emulsifying agent control and

- 1 for toxicity control.

 

The results of the test were as follows:

 

% biodegradation

Test item

(mean of replicates)

Reference item

(mean of replicates)

Toxicity Control

By day 28

16.8

98.9

52.1

By day 56

47.5

103.5

77.6

 

The test item was not readily biodegradable under the conditions of the test as it failed to achieve a transition from 10% to 60% degradation in a 10-day window during the 28 days test.

 

The test item was not considered to be inhibitory to the microbial inoculum since the biodegradation observed in the toxicity control bioreactor was not less than 25% occurred within 14 days.