Reaction mass of 3-[(4-amino-6-bromo-5,8-dihydro-1-hydroxy-8-imino-5-oxo-2-naphtyl)amino]-N,N,N-trimethylanilinium chloride and 3-[(8-amino-dibromo-5-hydroxy-4-imino-1-oxo-1,4-dihydronaphthalenyl)amino]-N,N,N-trimethylbenzenaminium chloride and 3-[(bromo-1,4-dihydroxy-8-imino-5-oxo-5,8-dihydronaphthalenyl)amino]-N,N,N-trimethylbenzenaminium chloride

PBT assessment

PBT assessment: overall result

PBT status:

the substance is not PBT / vPvB

Justification:

Persistence

Taking into account the lack of biodegradation in a ready biodegradation test (OECD 301B), Basic Blue 99 is considered as potentially persistent or very persistent on the basis of screening criteria.

Bioaccumulation

The octanol water partition coefficient log Kow of the main component was estimated to be -0.9. The octanol water partition coefficient of the components were estimated to range from -4.4 to 2.4. These low log Kow values indicate that the substance has hydrophilic properties and is not considered as bioaccumulable or very bioaccumulable.

Toxicity

Available data : EC50/algae/72 hours = 0.055 mg/L (OECD 201), EC50/daphnia magna/48 hours = 6.96 mg/L (OECD 202).

Criteria : Acute E(L)C50 from standard acute toxicity tests <0.1 mg/L.

The substance is not classified as carcinogenic (cat. 1A or 1B), nor germ cell mutagenic (Cat. 1A or 1B), nor toxic for the reproduction ((cat. 1A, 1B or 2). It is not classified as "Specific Target Organ Toxicity Repeated Exposure category 1 or category 2 ".

Screening assignment : T screening criteria met based on acute aquatic toxicity tests.

Basic Blue 99 is potentially Vp/P and Toxic but not B/vB. It is therefore not PBT or vP/vB.