Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 916-466-1
CAS number: -
Based on the geometric mean measured concentrations of the test item
Basic Blue 99, the 48 hour EC50 for Daphnia magna was 6.96 mg/L (95%
confidence limits : 6.04-9.11 mg/L).
In the acute immobilisation test with Daphnia magna, the effects of the
test item Basic Blue 99 were determined according to OECD guideline 202.
The study was conducted under semi-static conditions over a period of 48
hours with five concentrations of the test item Basic Blue 99 in the
range of 3.00 to 70.3 mg/L, prepared in a geometric series with a
separation factor of 2.2. All tested concentration levels were visually
clear at the start of the exposure and at the renewal after 24 hours and
exhibited a concentration dependent blue colouring from light blue
(lowest concentration) to dark blue (highest concentration). In the old
test media after 24 and 48 hours, test item particles were sedimented in
the three highest concentration levels (14.5 to 70.3 mg/L at 24 hours
and 6.60 to 31.9 mg/L at 48 hours). Twenty daphnids (divided into 4
replicates with 5 daphnids each) were exposed to each concentration
level and the control. The concentrations of the test item Basic Blue 99
were analytically verified via HPLC with diode array detection in fresh
media of all concentration levels and the control at the start of the
exposure and at renewal (0 and 24 hours) and in 24 hour old media at
renewal and at the end of the test (24 and 48 hours). The measured
concentrations of the test item in fresh media at the start of the
exposure and at renewal (0 and 24 hours) were in the range of 82 to 105%
of the nominal values and 66 to 102% in old media at renewal and at the
end of the test (24 and 48 hours). The effect concentrations are based
on geometric mean measured concentrations of the test item Basic Blue
99. The 48 hour EC50 for Daphnia magna was determined to be 6.96 mg/L
(95% confidence limits : 6.04-9.11 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again