Registration Dossier
Registration Dossier
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Reaction mass of 3-[(4-amino-6-bromo-5,8-dihydro-1-hydroxy-8-imino-5-oxo-2-naphtyl)amino]-N,N,N-trimethylanilinium chloride and 3-[(8-amino-dibromo-5-hydroxy-4-imino-1-oxo-1,4-dihydronaphthalenyl)amino]-N,N,N-trimethylbenzenaminium chloride and 3-[(bromo-1,4-dihydroxy-8-imino-5-oxo-5,8-dihydronaphthalenyl)amino]-N,N,N-trimethylbenzenaminium chloride
EC number: 916-466-1 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Based on the geometric mean measured concentrations of the test item Basic Blue 99, the 48 hour EC50 for Daphnia magna was 6.96 mg/L (95% confidence limits : 6.04-9.11 mg/L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 7 mg/L
Additional information
In the acute immobilisation test with Daphnia magna, the effects of the test item Basic Blue 99 were determined according to OECD guideline 202. The study was conducted under semi-static conditions over a period of 48 hours with five concentrations of the test item Basic Blue 99 in the range of 3.00 to 70.3 mg/L, prepared in a geometric series with a separation factor of 2.2. All tested concentration levels were visually clear at the start of the exposure and at the renewal after 24 hours and exhibited a concentration dependent blue colouring from light blue (lowest concentration) to dark blue (highest concentration). In the old test media after 24 and 48 hours, test item particles were sedimented in the three highest concentration levels (14.5 to 70.3 mg/L at 24 hours and 6.60 to 31.9 mg/L at 48 hours). Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration level and the control. The concentrations of the test item Basic Blue 99 were analytically verified via HPLC with diode array detection in fresh media of all concentration levels and the control at the start of the exposure and at renewal (0 and 24 hours) and in 24 hour old media at renewal and at the end of the test (24 and 48 hours). The measured concentrations of the test item in fresh media at the start of the exposure and at renewal (0 and 24 hours) were in the range of 82 to 105% of the nominal values and 66 to 102% in old media at renewal and at the end of the test (24 and 48 hours). The effect concentrations are based on geometric mean measured concentrations of the test item Basic Blue 99. The 48 hour EC50 for Daphnia magna was determined to be 6.96 mg/L (95% confidence limits : 6.04-9.11 mg/L).
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