Registration Dossier
Registration Dossier
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EC number: 277-539-5 | CAS number: 73570-52-2
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 15 Aug - 12 Sep 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Version / remarks:
- 1988
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3,7-bis(diethylamino)phenoxazin-5-ium acetate
- EC Number:
- 279-349-8
- EC Name:
- 3,7-bis(diethylamino)phenoxazin-5-ium acetate
- Cas Number:
- 79916-07-7
- Molecular formula:
- C20H26N3O.C2H3O2
- IUPAC Name:
- 3,7-bis(diethylamino)phenoxazin-5-ium acetate
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): ARA Rhein, Switzerland, collected on 14 Aug 1990
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
TEST SYSTEM
- Number of culture flasks/concentration: 2
SAMPLING
- Sampling frequency: day 0, 2, 5, 7, 9, 12, 14, 16, 19, 21, 23, 26 ,28
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
Reference substance
- Reference substance:
- diethylene glycol
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 33.5
- St. dev.:
- 0.2
- Sampling time:
- 28 d
- Remarks on result:
- other: adsorption after 3 h: 33.4%
- Details on results:
- The substance was mainly adsorbed to the sludge particles.
BOD5 / COD results
BOD5 / CODopen allclose all
- Parameter:
- COD
- Value:
- 1 360 other: mg/g O2
- Parameter:
- BOD5
- Value:
- 0 other: mg/g O2
- Remarks on result:
- not determinable
- Results with reference substance:
- 99.6% biodegradation (based on DOC removal) on day 12
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The substance is not inherently biodegradable. The mean degradation rate (based on DOC removal) was 33.5% on day 28. However, adsorption was found to be strong (ca. 33% after 3 h), so it can be assumed that the substance was mainly adsorbed by activated sludge particles. The measured COD and BOD5 do not indicate any potential for biodegradation either.
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