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EC number: 225-403-0 | CAS number: 4826-71-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- Guideline 202 on “Daphnia sp., Acute Immobilisation Test and Reproduction Test”, adopted in April 1984, included two parts: Part I - the 24h EC50 acute immobilisation test and Part II - the reproduction test (at least 14 days).
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Wako Pure Chemical Industries, Ltd., Lot No.: PAR1681.
- Purity: 100.2% - Analytical monitoring:
- yes
- Vehicle:
- yes
- Remarks:
- dechlorinated industrial water
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test substance was diluted with dilution water. Test substance was stored in freezer. The stability of the chemical was confirmed by IR absorption spectrum. Under the stock condition, IR spectrum of the test substance at the end of test was same at the start. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: National Institute of Environmental Studies (Japan). - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- Total hardness (as CaCO3): 27 mg/L
- Test temperature:
- 20 ± 1 ºC
- pH:
- 7.8
- Nominal and measured concentrations:
- Nominal: 3.2, 10, 32 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 80 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 58.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Remarks:
- Maximum
- Effect conc.:
- 30.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- >= 95.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 95.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- Since the test substance exceeded ± 20% of the nominal concentraion, calculation was performed using the time weighted average concentration.
- Reported statistics and error estimates:
- Probit method for EC50 if applicable. 1-way ANOVA (a=0.05) and Dunnett's method (a=0.05, both side) for NOEC, after Barlett's homoscedastic test.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 21 day-EC50 of choline chloride in Daphnia magna was 58.9 mg/L and the 21 day-NOEC was 30.2 mg/L.
- Executive summary:
A reproduction test on aquatic invertebrates was performed with choline chloride according to OECD Guideline 211 (OECD 202 adopted in April 1984), following GLP. The following test item concentrations 0 (control), 3.2, 10, 32 and 100 mg/L were tested in a semi-static system. Ten groups of ten daphnids were exposed to the different concentrations of the test item or the control for 21 days. After 21 days, the LC50 of Daphnia magna was 100 mg/L (nominal) and 95.5 mg/L (measured). The 21 day-EC50 of test item in Daphnia magna was 58.9 mg/L and the 21 day-NOEC was 30.2 mg/L.
Reference
Description of key information
The available data refer to the degradation product of calcium phosphorylcholine chloride:
- Key study. OECD 211. GLP study. The 21 day-EC50 of choline chloride in Daphnia magna was 58.9 mg/L and the 21 day-NOEC was 30.2 mg/L.
Based on this information, it can be concluded that the substance phosphoryl choline chloride has a 21day-NOEC in Daphnia magna above 30.2 mg/l.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 30.2 mg/L
Additional information
- Key study. A reproduction test on aquatic invertebrates was performed with choline chloride according to OECD Guideline 211 (OECD 202 adopted in April 1984), following GLP. The following test item concentrations 0 (control), 3.2, 10, 32 and 100 mg/L were tested in a semi-static system. Ten groups of ten daphnids were exposed to the different concentrations of the test item or the control for 21 days. After 21 days, the LC50 of Daphnia magna was 100 mg/L (nominal) and 95.5 mg/L (measured). The 21 day-EC50 of test item in Daphnia magna was 58.9 mg/L and the 21 day-NOEC was 30.2 mg/L.
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