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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1984
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Version / remarks:
Guideline 202 on “Daphnia sp., Acute Immobilisation Test and Reproduction Test”, adopted in April 1984, included two parts: Part I - the 24h EC50 acute immobilisation test and Part II - the reproduction test (at least 14 days).
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Wako Pure Chemical Industries, Ltd., Lot No.: PAR1681.
- Purity: 100.2%
Analytical monitoring:
yes
Vehicle:
yes
Remarks:
dechlorinated industrial water
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test substance was diluted with dilution water. Test substance was stored in freezer. The stability of the chemical was confirmed by IR absorption spectrum. Under the stock condition, IR spectrum of the test substance at the end of test was same at the start.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: National Institute of Environmental Studies (Japan).
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Hardness:
Total hardness (as CaCO3): 27 mg/L
Test temperature:
20 ± 1 ºC
pH:
7.8
Nominal and measured concentrations:
Nominal: 3.2, 10, 32 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 80 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark

Reference substance (positive control):
not specified
Key result
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
58.9 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Key result
Duration:
21 d
Dose descriptor:
NOEC
Remarks:
Maximum
Effect conc.:
30.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
>= 95.5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 95.5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
Since the test substance exceeded ± 20% of the nominal concentraion, calculation was performed using the time weighted average concentration.
Reported statistics and error estimates:
Probit method for EC50 if applicable. 1-way ANOVA (a=0.05) and Dunnett's method (a=0.05, both side) for NOEC, after Barlett's homoscedastic test.
Validity criteria fulfilled:
not specified
Conclusions:
The 21 day-EC50 of choline chloride in Daphnia magna was 58.9 mg/L and the 21 day-NOEC was 30.2 mg/L.
Executive summary:

A reproduction test on aquatic invertebrates was performed with choline chloride according to OECD Guideline 211 (OECD 202 adopted in April 1984), following GLP. The following test item concentrations 0 (control), 3.2, 10, 32 and 100 mg/L were tested in a semi-static system. Ten groups of ten daphnids were exposed to the different concentrations of the test item or the control for 21 days. After 21 days, the LC50 of Daphnia magna was 100 mg/L (nominal) and 95.5 mg/L (measured). The 21 day-EC50 of test item in Daphnia magna was 58.9 mg/L and the 21 day-NOEC was 30.2 mg/L.

Description of key information

The available data refer to the degradation product of calcium phosphorylcholine chloride:

- Key study. OECD 211. GLP study. The 21 day-EC50 of choline chloride in Daphnia magna was 58.9 mg/L and the 21 day-NOEC was 30.2 mg/L.

Based on this information, it can be concluded that the substance phosphoryl choline chloride has a 21day-NOEC in Daphnia magna above 30.2 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
30.2 mg/L

Additional information

- Key study. A reproduction test on aquatic invertebrates was performed with choline chloride according to OECD Guideline 211 (OECD 202 adopted in April 1984), following GLP. The following test item concentrations 0 (control), 3.2, 10, 32 and 100 mg/L were tested in a semi-static system. Ten groups of ten daphnids were exposed to the different concentrations of the test item or the control for 21 days. After 21 days, the LC50 of Daphnia magna was 100 mg/L (nominal) and 95.5 mg/L (measured). The 21 day-EC50 of test item in Daphnia magna was 58.9 mg/L and the 21 day-NOEC was 30.2 mg/L.