2,4-dichlorophenol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1985

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The SIDS document contains the information gathered and an initial assessment performed under the framework of the OECD HPV Chemicals Programme. The information is provided as an indication of the current state of knowledge of this substance based on the Screening Information Data Set (SIDS), but does not presume to be comprehensive. The SIDS document for 2,4-Dichlorophenol has been peer reviewed by the French government and was performed by the National Institute on Environmental and Industrial Risk (INERIS) by request from the Ministry of Environment.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not specified

Principles of method if other than guideline:

No additional details presented

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dichlorophenol

EC Number:

204-429-6

EC Name:

2,4-dichlorophenol

Cas Number:

120-83-2

Molecular formula:

C6H4Cl2O

IUPAC Name:

2,4-dichlorophenol

Details on test material:

The purity of the substance (purchased from Aldrich) is reported as 99%

Test animals

Species:

mouse

Strain:

CD-1

Sex:

male/female

Details on test animals or test system and environmental conditions:

Doses were administered by gavage to 6 week-old male and female CD-1 ICR mice (8 animals/sex/group; 5 dose levels, 1 animal/cage). Temperature was maintained at : 22°+/-2°C, Humidity at: 40-60% and the Light cycle was : 12h/d.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

10 ml/kg were administered by gavage to 6 week-old male and female CD-1 ICR mice (8 animals/sex/group; 5 dose levels, 1 animal/cage).

Doses:

5 dose levels were administered

No. of animals per sex per dose:

Eight

Control animals:

not specified

Details on study design:

LD50 is calculated by probit analysis and Litchfield-Wilcoxon test.

Results and discussion

Preliminary study:

No information on a preliminary study is reported.

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 276 - < 1 352 mg/kg bw

Based on:

test mat.

Mortality:

All deaths occurred within 6-24 hours after dosing.

Clinical signs:

other: All animals displayed signs evocating CNS disorders within 10-15 min after dosing: ataxia, loss of righting reflex, slight tremors, salivation, laboured breathing and depression.

Gross pathology:

At necropsy, no gross abnormalities were noted in dead animals nor in survivors after a 2-week observation period.

Other findings:

None

Any other information on results incl. tables

No additional information presented

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 was determined (in mice [male/female]) in a study similar to OECD guideline 401. The results indicate the LD50 to range between 1276 and1352 mg/kg bw.

Executive summary:

An LD50 study was conducted in male and female mice using a method similar to OECD guideline 401.

The substance purity is reported as 99% (as procured from Aldrich) at the beginning of the study.

In the course of the study clinical signs noted included : CNS disorders within 10-15 min after dosing: ataxia, loss of righting reflex, slight tremors, salivation, laboured breathing and depression.

The results indicate the LD50 to range between 1276 and 1352 mg/kg bw.

This study is assigned a reliablility of 1, valid without restriction in the SIDS document