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EC number: 268-717-3 | CAS number: 68133-90-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Endpoint summary
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- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
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- Terrestrial toxicity
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- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- GLP-status unknown, adequate for assessment, published in peer-reviewed literature
Data source
Reference
- Reference Type:
- publication
- Title:
- Impact of platinum group elements on the soil invertebrate Enchytraeus crypticus
- Author:
- Havelkova. B et al.
- Year:
- 2 014
- Bibliographic source:
- Neuroendocrinology Letters 35 (2) 101–108
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 220 (Enchytraeid Reproduction Test)
- Deviations:
- yes
- Remarks:
- The test duration was reduced to 28 days, because of the smaller size and shorter reproductive cycle of E. crypticus
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Tetrachloroplatinum
- Cas Number:
- 13454-96-1
- Molecular formula:
- PtCl4
- IUPAC Name:
- Tetrachloroplatinum
Constituent 1
- Specific details on test material used for the study:
- TEST MATERIAL: Sigma-Aldrich PtCl4, purity 99.99 %
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): The soil was contaminated by 0.084225 g test item dissolved in 250 mL deionised water to achieve soil moisture equal to 50 % of maximum water holding capacity.
- Controls: treatment without the test item
Test organisms
- Test organisms (species):
- Enchytraeus crypticus
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Source: cultures from the Ecotoxicological laboratory of the University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic
- Age of test organisms: The worms used in the test were adult with eggs in the clitellum region and they were of approximately of the same size
- Feeding: Finely ground rolled oats. Additional food and water were added weekly, as necessary
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 20 ± 2 °C
- Details on test conditions:
- TEST SYSTEM
- Amount of soil or substrate: 20 g per test vessel
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 5
- No. of replicates per control: 5
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture
- % sand: 70
- % peat: 10 - finely ground Sphagnum peat
- % kaolin clay: 20
- pH: 6.0 ± 0.5
- Interval of measurements: pH and moisture were measured at the beginning and at the end of the test using an extra replicate without organisms
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark
EFFECT PARAMETERS MEASURED: mortality was measured at the beginning and at the end of the test. the number of juveniles produced was also recorded throughout the test.
VEHICLE CONTROL PERFORMED: no
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ranged from 1.25 to 2
- Range finding study: yes - Nominal and measured concentrations:
- Nominal concentrations: 50, 100, 150, 200 and 250 μmol test item/L
- Reference substance (positive control):
- yes
- Remarks:
- Boric acid
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 161.9 other: μmol/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 93.75 other: μmol/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- reproduction
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 54.54 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 31.58 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- reproduction
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 300 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Basis for effect:
- reproduction
- Remarks on result:
- other: EC10 value calculated based on reported results using TRAP v1.30a
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 695 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Basis for effect:
- reproduction
- Remarks on result:
- other: EC50 value calculated based on reported results using TRAP v1.30a
- Details on results:
- - No significant effect on mortality was found after 28 days of exposure to the test item.
- All Coefficient Variation (CV) values were less than 32 % for each test item concentration.
- Mean adult survival rates ranged from 8 to 10 out of 10 enchytraeids per vessel. - Results with reference substance (positive control):
- - The 28-d EC50 of boric acid was found to be 156.2 mg/kg.
- In the control, adult survival was 100 %, the average number of juveniles per vessel was 199.6 and the coefficient of variation (CV) was 6 % in juveniles.
- in the treatment, the vaerage number of juveniles per vessel ranged from 58.6 to 139.2 and all CV values were less than 21 % for each concentration of boric acid. The inhibition of reproduction ranged from 30 to 71 %. Mean adult survival rates ranged from 6 to 10 out of 10 animals per test vessel. - Reported statistics and error estimates:
- Levene's method was used to test for the homogeneity of variances. Non-homogenous parameters were log-transformed prior to analysis and compared with the non-parametric Kruskal-Wallis test. All tests were considered statistically significant and highly significant when resulting in values of p < 0.05 and p < 0.01, respectively.
Any other information on results incl. tables
Calculation EC10&EC50 (in mg/kg(dw))
Enchytraeus sp | |||
conc (µmol/L) as PtCl4 | n° juveniles | repr inhib | |
control | 130 | ||
50 | 130 | 0 | |
100 | 105 | 19 | |
150 | 69.2 | 47 | |
200 | 57.2 | 56 | |
250 | 30.4 | 77 | |
reported | 28d EC50 | 161.9 µmol/L | |
recalculated | EC10 | 71.4 | |
EC50 | 165.3 |
PtCl4 (휇mol/L) | Pt (휇mol/L) | Pt (휇g/L) | Pt (mg/L) | Pt (mg/kg ww) | Pt (mg/kg dw) | ||
EC10 | 71.4 | 41.3 | 8065.6 | 8.1 | 265.7 | 300.2 | |
EC50 | 165.3 | 95.7 | 18672.8 | 18.7 | 615.1 | 695.1 | |
MW PtCl4 | 336.89 | 1.726933 | |||||
MW Pt | 195 | ||||||
Kd soil | 37.2 | L/kg | |||||
Ksoil-water | 56 | m3/m3 | |||||
RHOsolid | 1700 | kg/m3 | |||||
Dry weight conversion | 1.13 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 28-d EC50 of platinum(IV) chloride for inhibition of the reproduction tof Enchytraeus crypticus was determined to be 161.9 μmol test item/L. Recalculation of effects concentration using TRAP v1.30a gave EC10 and EC50 values of 71.4 and 165.3 µmol Pt/L, respectively, corresponding to 300 and 695 mg Pt/kg(dw), respectively.
- Executive summary:
The chronic toxicity of platinum (as platinum (IV) chloride) to the soil invertebrate Enchytraeus crypticus was assessed in a publication (Havelkova et al. 2014). The study is reliable with acceptable restrictions, with an unknown GLP status, but adequate for assessment. The test guideline OECD 220 was followed.
A laboratory test of 28 days with artificial soil was carried out to determine the effects of platinum on the reproduction of E. crypticus. Five nominal concentrations of the test item were tested, alongside a negative control and a reference control. No significant effect on mortality was found. The 28-d EC50 of platinum(IV) chloride for inhibition of the reproduction of Enchytraeus crypticus was determined to be 161.9 μmol test item/L (93.75 μmol Pt/L). Recalculation of effects concentration using TRAP v1.30a gave EC10 and EC50 values of 71.4 and 165.3 µmol Pt/L, respectively, corresponding to 300 and 695 mg Pt/kg(dw), respectively.
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