6,10,14-trimethylpentadecan-2-one

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Test type:

other: inhalation hazard test

Test material

Test material information

Constituent 1

Reference substance name:

6,10,14-trimethylpentadecan-2-one

EC Number:

207-950-7

EC Name:

6,10,14-trimethylpentadecan-2-one

Cas Number:

502-69-2

Molecular formula:

C18H36O

IUPAC Name:

6,10,14-trimethylpentadecan-2-one

Details on test material:

- name of test substance: Hexahydrofarnesylaceton
- batch number: continuous production
- purity: 93.4%
- date of manufacture: Apr. 20, 1988
- physical state / appearance: liquid / colorless
- storage conditions: was stored at room temperature
- stability: at least for the study period under normal conditions
- administration form: unchanged

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Strain: SPF Wistar / Chbb: THOM
- Source: Dr. K. Thomae GmbH, 7950 Biberach, Germany
- Age at study initiation: about 8-9 weeks
- Weight at study initiation: male: 291 g, female: 195 g
- Identification: dye marks at tail
- Housing: in threes in wire mesh cage (Becker, D III), no bedding
- Diet (e.g. ad libitum): KLIBA 24-343-4 rat/mouse laboratory diet, 10 mm pellets, Klingenthalmühle AG, CH-4303 Kaiseraugst, ad libitum in the period without exposure
- Water (e.g. ad libitum): ad libitum in the period without exposure
- Acclimation period: at least 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12 / 12

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

Generation of the atmosphere for inhalation; exposure
The product was introduced to a height of 5 cm into a glass bottle (generator) with a sintered glass disk (pore-size 90 - 150 µm, diameter 30 mm), and the weight was determined .
The generator containing the product was placed in a waterbath maintained at 20°C by a thermostat, and a stream of 200 l/h compressed air was supplied to a downstream mixing chamber . The mixture of air and test substance generated in this way was passed through a glass distributor to 6 glass tubes in which 3 male and 3 female animals had been placed . The emerging mixtures of test substance and air were exhausted.
Suitable measures were applied to ensure that the air supply had the same temperature as in the laboratories, so that the temperature in the exposure apparatus was between 19 and 25°C . There were no deviations from these set conditions which might have adversely affected the results of the study.
After 30 minutes, the generator was replaced by a new one containing fresh test substance as described above. This generator was then used for the remainder of the test.
The exposure time was 7 hours . The intention was to determine the exposure time which was survived, together with an observation period lasting 14 days, by all the animals.

Duration of exposure:

7 h

Concentrations:

0.43 mg/l

No. of animals per sex per dose:

3

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical examination: each work day, lethality checked each day
- Necropsy of survivors performed: yes

Results and discussion

Mortality:

none during 7 hrs in a saturated atmosphere at 20°C or 14 days observation period

Clinical signs:

other: During exposure : accelerated respiration, snout wiping, snout licking After exposure : accelerated respiration, urine-contaminated fur . Form day 1 of the observation period onward, no abnormalities were detected in the animals .

Gross pathology:

Sacrificed animals: male + female
No pathologic findings noted

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification