6,10,14-trimethylpentadecan-2-one

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

Method: other: BASF-Test

GLP compliance:

no

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

6,10,14-trimethylpentadecan-2-one

EC Number:

207-950-7

EC Name:

6,10,14-trimethylpentadecan-2-one

Cas Number:

502-69-2

Molecular formula:

C18H36O

IUPAC Name:

6,10,14-trimethylpentadecan-2-one

Details on test material:

Purity: unknown
Density: 0.834 g/ml

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, 7950 Biberach, Germany
- Weight at study initiation: male: 268 g, female: 238 g
- Identification: cage cards
- Housing: single housing in stainless steel wire mesh cage (type DK-III, Becker & Co., Castrop-Rauxel, Germany)
- Diet (e.g. ad libitum): KLIBA-Labordiät 343, Klingenthalmühle AG, CH-4303 Kaiseraugst, ad libitum
- Water (e.g. ad libitum): tap water ad libitum
- Acclimation period: at least 1 week


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12 / 12

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

- application area: about 50 cm²
- route of application: single application to the clipped epidermis (dorsal and dorsolateral parts of the trunk)
- clipping of the fur: at least 15 hours before beginning of thy study
- application: in the morning, Aug. 25, 1988
- coverage of the application site with a semiocclusive dressing (four layers of absorbent gauze and porous bandage) for 24 hours
- afterwards: removal of the dressing and rinsing of the application site with warm water

Duration of exposure:

24 h

Doses:

2000 mg/kg bw (= 2,4 ml/kg bw)

No. of animals per sex per dose:

5

Details on study design:

- Duration of observation period following administration: 14 days
- signs and symptoms: recording several times on the day of application and then at least once each workday
- check for moribund and dead animals: twice each workday and once on holidays
- scoring of skin findings: 30-60 min after removal of the semiocclusive dressing and then about one week later and before termination of the study
- pathology: withdrawal of food about 16 hours before sacrifice with CO2; then necropsy with gross-pathological examination; necropsy of all animals that die as early as possible

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

none during 24 hours exposure and 14 day observation period

Clinical signs:

symptoms: no abnormalities
erythema: only on day 1 in males and females

Body weight:

mean weight male [g]:
268 day 0
293 day 7
317 day 13

mean weight female [g]:
238 day 0
243 day 7
253 day 13

Gross pathology:

sacrificed animals (male+female): no pathological findings noted

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met