(carboxylatomethyl)dodecyldimethylammonium

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: Publication

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

review article or handbook

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

OECD. 202 Daphnia sp. Acute Immobilisation Test OECD Guidelines for Testing of
Chemicals. Paris; 2004.

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

(carboxylatomethyl)dodecyldimethylammonium

EC Number:

211-669-5

EC Name:

(carboxylatomethyl)dodecyldimethylammonium

Cas Number:

683-10-3

Molecular formula:

C16H33NO2

IUPAC Name:

(carboxylatomethyl)dodecyldimethylammonium

Test material form:

not specified

Details on test material:

Unknown

Specific details on test material used for the study:

The amphoteric surfactants tested were: three alkyl betaines (decyl dimethyl-,dodecyl dimethyl- and tetradecyl dimethyl betaine; C10-Bet, C12-Bet and C14-Bet,
respectively). The alkyl betaines were supplied by Albright and Wilson Ltd.
The lauryl betaine substance, subject of this registration, is a predominently C12 alkyl betaine (97%). Use of data from this scientific paper is justified since the C12 alkyl betaine used for the investigation of the environmental effects is similar to this substance

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

D. magna, laboratory bred, not more than 24 h old were used in this test

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/l as CaCO3

Test temperature:

20C

pH:

8

Details on test conditions:

Testswere performed in the dark at 20 °C. Twenty daphnia, divided into four batches of five animals each,was used at each test concentration. For each surfactant, ten concentrations in a geometric series
were tested in the concentration range first established in a preliminary test. The percentage immobility at 48 h was plotted against concentration on logarithmic probability paper and a linear relationship was obtained.
The ProbitMethodwas employed as the statistical procedure to determine the IC50 (the estimated concentration to immobilise 50% of the daphnia after 48 h exposure) and the corresponding 95% confidence interval (CI).

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

5.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The conclusion of this study was that Alkyl betaines (C12-C14) were found to be Moderately toxic to aqautic life.
However, in addition, the biodegradation curves obtained for the alkyl betaines tested showed a high
biodegradation level (>60%) was reached by the end of the test according to the requirements of OECD 202