3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1)

Endpoint summary

Description of key information

Skin irritation:

The dermal irritation potential of 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)was estimated using prediction model OECD QSAR toolbox version 3.3 and the data available forits closest read across substances with log kow as the primary descriptor. Thus based on the predictions as well as its read across substances and applying weight of evidence, it can be concluded that the chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) is not irritating to skin and can be classified under the category ˋ Not Classified’ as per CLP regulation.

 

Eye irritation:

The ocular irritation potential of 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) was estimated using prediction model OECD QSAR toolbox version 3.3 and the data available forits closest read across substances with log kow as the primary descriptor. Thus based on the predictions as well as its read across substances and applying weight of evidence, it can be concluded that the chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) is severely irritating to the eyes and can be classified under the category ˋ Category 1’ as per CLP regulation.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification

Justification for type of information:

Data is from OECD QSAR toolbox version 3.3 and QMRF report has been attached

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Prediction is done using QSAR Toolbox version 3.3

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1)
- Molecular formula: C40H52Cl4N6O2Zn
- Molecular weight: 856.093 g/mol
- Smiles notation: [o+]1c2cc(ccc2nc2ccc(N(CC)CC)cc12)N(CC)CC.[Zn+2].[ClH-].[ClH-].[ClH-].[ClH-].[o+]1c2cc(ccc2nc2c1cc(cc2)N(CC)CC)N(CC)CC
- InChl: 1S/2C20H26N3O.4ClH.Zn/c2*1-5-22(6-2)15-9-11-17-19(13-15)24-20-14-16(23(7-3)8-4)10-12-18(20)21-17;;;;;/h2*9-14H,5-8H2,1-4H3;4*1H;/q2*+1;;;;;+2/p-4
- Substance type: Organic
- Physical state: Solid

Details on test system:

No data available

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

No data available

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

No data available

Duration of treatment / exposure:

4 hours

Observation period:

72 hours

Number of animals:

3

Details on study design:

No data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

72 h

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No skin sensitization was observed in treated rabbits

Estimation method: Takes mode value from the 8 nearest neighbours
Domain  logical expression:Result: In Domain

(((((("a" or "b" or "c" )  and ("d" and ( not "e") )  )  and ("f" and ( not "g") )  )  and "h" )  and ("i" and ( not "j") )  )  and ("k" and "l" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as Zinc metal and salts by OECD HPV Chemical Categories

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Soluble complexes of Zinc by US-EPA New Chemical Categories

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Tertiary aromatic amine by DNA binding by OECD

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as No alert found by Protein binding by OASIS v1.3

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Acylation OR Acylation >> Ester aminolysis OR Acylation >> Ester aminolysis >> Dithiocarbamates OR SN1 OR SN1 >> Carbenium ion formation (enzymatic) OR SN1 >> Carbenium ion formation (enzymatic) >> Carbenium ion OR SN2 OR SN2 >> Nucleophilic substitution at sp3 carbon atom OR SN2 >> Nucleophilic substitution at sp3 carbon atom >> Alkyl halides  OR SN2 >> Ring opening SN2 reaction OR SN2 >> Ring opening SN2 reaction >> Epoxides, Aziridines and Sulfuranes  by Protein binding by OASIS v1.3

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as Aromatic mono- and dialkylamine AND H-acceptor-path3-H-acceptor by in vivo mutagenicity (Micronucleus) alerts by ISS

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as 1-phenoxy-benzene OR Aromatic diazo OR Hydrazine OR Nitro-aromatic OR No alert found OR Oxolane by in vivo mutagenicity (Micronucleus) alerts by ISS

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as Not bioavailable by Lipinski Rule Oasis ONLY

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as Halogens AND Non-Metals AND Transition Metals by Groups of elements

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as Alkali Earth by Groups of elements

Domain logical expression index: "k"

Parametric boundary:The target chemical should have a value of log Kow which is >= 3.22

Domain logical expression index: "l"

Parametric boundary:The target chemical should have a value of log Kow which is <= 14.5

Interpretation of results:

other: not irritating

Conclusions:

The chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)can be considered to be not irritating to skin.

Executive summary:

The dermal irritation potential of 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)was estimated using OECD QSAR toolbox version 3.3 with logPow as the primary descriptor. The chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)can be considered to be not irritating to skin and can be classified under the category ˋ Not Classified’ as per CLP regulation.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification

Justification for type of information:

Data is from OECD QSAR toolbox version 3.3 and QMRF report has been attached

Qualifier:

according to guideline

Guideline:

other: estimated data

Principles of method if other than guideline:

Prediction is done using QSAR Toolbox version 3.3

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1)
- Molecular formula: C40H52Cl4N6O2Zn
- Molecular weight: 856.093 g/mol
- Smiles notation: [o+]1c2cc(ccc2nc2ccc(N(CC)CC)cc12)N(CC)CC.[Zn+2].[ClH-].[ClH-].[ClH-].[ClH-].[o+]1c2cc(ccc2nc2c1cc(cc2)N(CC)CC)N(CC)CC
- InChl: 1S/2C20H26N3O.4ClH.Zn/c2*1-5-22(6-2)15-9-11-17-19(13-15)24-20-14-16(23(7-3)8-4)10-12-18(20)21-17;;;;;/h2*9-14H,5-8H2,1-4H3;4*1H;/q2*+1;;;;;+2/p-4
- Substance type: Organic
- Physical state: Solid

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

not specified

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

No data available

Duration of treatment / exposure:

single application

Observation period (in vivo):

72 hours

Number of animals or in vitro replicates:

3

Details on study design:

No data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

72 h

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

Severe eye irritation was observed in treated rabbits.

Estimation method: Takes mode value from the 5 nearest neighbours
Domain  logical expression:Result: In Domain

(((((((((("a" or "b" or "c" )  and ("d" and ( not "e") )  )  and ("f" and ( not "g") )  )  and ("h" and ( not "i") )  )  and ("j" and ( not "k") )  )  and "l" )  and ("m" and ( not "n") )  )  and ("o" and ( not "p") )  )  and "q" )  and ("r" and "s" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as Zinc metal and salts by OECD HPV Chemical Categories

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Soluble complexes of Zinc by US-EPA New Chemical Categories

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Tertiary aromatic amine by DNA binding by OECD

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as No alert found by DNA binding by OASIS v.1.3

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Non-covalent interaction OR Non-covalent interaction >> DNA intercalation OR Non-covalent interaction >> DNA intercalation >> Fused-Ring Primary Aromatic Amines OR Non-specific OR Non-specific >> Incorporation into DNA/RNA, due to structural analogy with  nucleoside bases    OR Non-specific >> Incorporation into DNA/RNA, due to structural analogy with  nucleoside bases    >> Specific Imine and Thione Derivatives OR Radical OR Radical >> Radical mechanism via ROS formation (indirect) OR Radical >> Radical mechanism via ROS formation (indirect) >> Fused-Ring Primary Aromatic Amines OR Radical >> Radical mechanism via ROS formation (indirect) >> Hydrazine Derivatives OR Radical >> Radical mechanism via ROS formation (indirect) >> Nitro Azoarenes OR Radical >> Radical mechanism via ROS formation (indirect) >> Specific Imine and Thione Derivatives OR SN1 OR SN1 >> Nucleophilic attack after metabolic nitrenium ion formation OR SN1 >> Nucleophilic attack after metabolic nitrenium ion formation >> Fused-Ring Primary Aromatic Amines OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation >> Nitro Azoarenes OR SN1 >> Nucleophilic substitution on diazonium ions OR SN1 >> Nucleophilic substitution on diazonium ions >> Specific Imine and Thione Derivatives OR SN2 OR SN2 >> Alkylation, direct acting epoxides and related OR SN2 >> Alkylation, direct acting epoxides and related >> Epoxides and Aziridines OR SN2 >> Alkylation, direct acting epoxides and related after cyclization OR SN2 >> Alkylation, direct acting epoxides and related after cyclization >> Nitrogen Mustards by DNA binding by OASIS v.1.3

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as Non binder, MW>500 by Estrogen Receptor Binding

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as Non binder, non cyclic structure OR Non binder, without OH or NH2 group OR Strong binder, OH group OR Weak binder, OH group by Estrogen Receptor Binding

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as No alert found by Protein binding by OASIS v1.3

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as Michael Addition OR Michael Addition >> Polarised Alkenes OR Michael Addition >> Polarised Alkenes >> alpha,beta-Unsaturated oximes  OR SN1 OR SN1 >> Carbenium ion formation (enzymatic) OR SN1 >> Carbenium ion formation (enzymatic) >> Carbenium ion by Protein binding by OASIS v1.3

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as No alert found by Protein binding by OECD

Domain logical expression index: "k"

Referential boundary: The target chemical should be classified as Acylation OR Acylation >> Direct Acylation Involving a Leaving group OR Acylation >> Direct Acylation Involving a Leaving group >> Acetates by Protein binding by OECD

Domain logical expression index: "l"

Referential boundary: The target chemical should be classified as No superfragment by Superfragments ONLY

Domain logical expression index: "m"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg AND Group All Aqueous Solubility < 0.000005 g/L AND Group All Aqueous Solubility < 0.00002 g/L AND Group All log Kow > 9 AND Group All Melting Point > 200 C AND Group All Molecular Weight > 650 g/mol by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "n"

Referential boundary: The target chemical should be classified as (!Undefined)Group All log Kow < -3.1 OR (!Undefined)Group All log Kow > 9 OR (!Undefined)Group All Melting Point > 200 C OR (!Undefined)Group CN Lipid Solubility < 0.4 g/kg OR Group All log Kow < -3.1 OR Group CN Aqueous Solubility < 0.1 g/L OR Group CN log Kow > 4.5 OR Group CN Molecular Weight > 290 g/mol OR Group CNS Aqueous Solubility < 0.006 g/l OR Group CNS log Kow > 1.5 OR Group CNS Melting Point > 200 C OR Group CNS Melting Point > 50 C OR Group CNS Molecular Weight > 620 g/mol by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "o"

Referential boundary: The target chemical should be classified as Halogens AND Non-Metals AND Transition Metals by Groups of elements

Domain logical expression index: "p"

Referential boundary: The target chemical should be classified as Alkali Earth by Groups of elements

Domain logical expression index: "q"

Referential boundary: The target chemical should be classified as Amine AND Anion AND Aromatic compound AND Cation AND Tertiary amine AND Tertiary mixed amine by Organic functional groups, Norbert Haider (checkmol) ONLY

Domain logical expression index: "r"

Parametric boundary:The target chemical should have a value of log Kow which is >= 8.68

Domain logical expression index: "s"

Parametric boundary:The target chemical should have a value of log Kow which is <= 16.6

Interpretation of results:

Category 1 (irreversible effects on the eye)

Conclusions:

The chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)was estimated to be not irritating into the eyes of New Zealand White rabbits. Based on the estimated result 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)can be considered to be severely irritating to eye.

Executive summary:

The ocular irritation potential of 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)was estimated using OECD QSAR toolbox version 3.3 with logPow as the primary descriptor. The chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)was estimated to be not irritating into the eyes of New Zealand White rabbits. Based on the estimated result 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)can be considered to be severely irritating to eye and can be classified under the category ˋ Category 1’ as per CLP regulation.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed (irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin irritation:

Various studieshas been investigated for the test chemical3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9)to observe the potential for dermal irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits for target chemical3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) and its closest read across substances with log kow as the primary descriptorN, N-dimethyl-p-toluidine (CAS No.:99-97-8)and Benzoic acid, 2-[4-(diethylamino)-2-hydroxybenzoyl]-, hexyl ester (CAS No: 302776-68-7).The predicted data using the OECD QSAR toolbox has also been compared with the experimental data and summarized as below;

 

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the skin irritation potential was estimated for test chemical3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) .The chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) is estimated to be not irritating to skin of New Zealand White rabbits.

 

A study was designed and conducted by Sustainability Support Services (Europe) AB, (2017) to determine the dermal reaction profile of read across chemical “N, N-dimethyl-p-toluidine (CAS No.:99-97-8)” in Rabbits that supports the above mentioned result. The study was performed as per OECD Guidelines 404 and complying to the GLP procedures. Three healthy young adult female rabbits were used for conducting acute dermal irritation study. Body weights were re­corded on day 0 (prior to application) and at termination. Rabbits with good intact skin were selected for the study. The hairs of all the rabbits were clipped at contralateral sites, approximately 24 hours prior to treatment. A dose of0.5 ml of test item (as such) was applied to the skin, over an area of approximately 6 x 6 cm clipped of hair on one side of rabbits. The other untreated side was kept as control area and 0.5 ml of distilled water was applied at this site. At the end of 4 hours, the gauze patch was removed and test item application site was wiped with water without altering the integrity of the epidermis. Initially, the test item was applied to the clipped area of skin of one rabbit. The test site was covered with gauze patch. After 4 hours of exposure in Animal No. 1, there was very slight erythema (barely perceptible) and very slight oedema (barely perceptible) observed at 1 hour of observation. At 24 hour very slight erythema (barely perceptible) and no oedema was observed.Hence the confirmatory test was conducted after 24 hours on additional two rabbits (No. 2 and 3)to confirm the non-irritant nature of the test item. Animal No. 2, revealed very slight erythema (barely perceptible) and very slight oedema (barely perceptible) observed at 1 hour of observation post patch removal. At 24 hour well defined erythema and very slight oedema (barely perceptible) was observed. At 48 hour very slight erythema (barely perceptible) and no oedema was observed. At 72 hour no erythema and no oedema was observed. Animal No. 3, revealed very slight erythema (barely perceptible) and very slight oedema (barely perceptible) observed at 1 hour of observation post patch removal. At 24 hour slight erythema (barely perceptible) and no oedema was observed. At 48 and 72 hour no erythema and no oedema was observed. The individual mean score at 24, 48 and 72 hours for animal nos. 1, 2 and 3 were 0.33, 1.00, 0.33 and 0.00, 0.33, 0.00, for erythema and oedema formation, respectively. Hence, it was concluded that “N, N-dimethyl-p-toluidine (CAS No. – 99-97-8)” was Non-Irritating to the skin of Female New Zealand White rabbits under the experimental conditions tested.

 

The above results were further supported by the skin irritation study reported by European Commission (EC) - Scientific Committee on Consumer Products (SCCP) { Opinion on: Diethylamino Hydroxybenzoyl Hexyl Benzoate (S83); European Commission (EC) - Scientific Committee on Consumer Products (SCCP); SCCP/1166/08; 15 April 2008} for read across substance Benzoic acid, 2-[4-(diethylamino)-2-hydroxybenzoyl]-, hexyl ester (CAS No: 302776-68-7) on New Zealand White rabbits in accordance with OECD 404.In this study, the chemical was applied to intact skin of each rabbits for the exposure period of 4 hours under semi-occlusive condition and skin reactions were observed for 24 and 72 hours.Slight erythema was observed in 2 animals on the day of application. No oedema was observed. The third animal did not show any skin reactions. The cutaneous reactions were reversiblein the animals within 48 hours after removal of the patch at latest. The average score (24 to 72 hours) for irritation was calculated to be 0.1 for erythema and 0.0 for oedema.Since the observed cutaneous effects were not persisted, the chemicalBenzoic acid, 2-[4-(diethylamino)-2-hydroxybenzoyl]-, hexyl ester (CAS No: 302776-68-7) was considered to benot irritating to the skin ofNew Zealand Whiterabbits.

 

 

Thus on the basis of available data for thetarget chemical3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) and its closest read across substance with log kow as the primary descriptorN, N-dimethyl-p-toluidine (CAS No.:99-97-8)and Benzoic acid, 2-[4-(diethylamino)-2-hydroxybenzoyl]-, hexyl ester (CAS No: 302776-68-7),it can be concluded thatchemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) is unable to cause skin irritation and considered as not irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.

 

 

Eye irritation:

In different studies,the test chemical3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) has been investigated for potential for ocular irritationto a greater or lesser extent. The studies are based on in vivo experiments in rabbits for target chemical3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) andits closest read across substance with log kow as the primary descriptorbis (2-ethylhexyl) zinc dithiophosphate (CAS No: 4259-15-8) and Zinc bis (O, O-bis (1, 3-dimethylbutyl) dithiophosphate(CAS No: 2215–35–2).The predicted data using the OECD QSAR toolbox has also been compared with the experimental data and summarized as below;

 

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the eye irritation potential was estimated for test chemical3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) .The chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) is estimated to be irritating to eye of New Zealand White rabbits.

 

The MAK Collection for Occupational Health and Safety {Bis(2-ethylhexyl)zinkdithiophosphat [MAK Value Documentation in German language, 2004]. The MAK Collection for Occupational Health and Safety. 1–14. 2012} conducted aneye irritation study of read across chemicalbis (2-ethylhexyl) zinc dithiophosphate (CAS No: 4259-15-8) in accordance with OECD Test Guideline 405.During this test, theundiluted test chemicalwas instilled into eyes of each rabbits. The rabbits were observed and scored for signs of irritation at 96 hours. The meanPrimary irritation indexscores after 96 hour was 23.5 out of maximum score of 110.Hence,bis (2-ethylhexyl) zinc dithiophosphate (CAS No: 4259-15-8)can be considered to irritating to rabbit eyes.

 

The above results were supported by the experimental report reported by EUROPEAN COMMISSION – European Chemicals Bureau {IUCLID DATABSE- EUROPEAN COMMISSION – European Chemicals Bureau- EUROPEAN COMMISSION – 18–FEB–2000} for read across substance Zinc bis (O, O-bis (1, 3-dimethylbutyl) dithiophosphate(CAS No: 2215–35–2) in accordance with OECD Test Guideline 405.The undiluted chemical was installed into the eye of rabbits and reactions were observed at 24, 48 and 72 hours after installation. The treated rabbits showed corrosive eye effects. Hence the chemicalchemicalZinc bis (O, O-bis (1, 3-dimethylbutyl) dithiophosphate(CAS No: 2215–35–2) was considered to be irritating to the rabbits’ eyeby FHSA criteria; meets EEC (R36) criteria for irritation.

 

Thus on the basis of available data for thetarget chemical3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) and its closest read across substance with log kow as the primary descriptorbis (2-ethylhexyl) zinc dithiophosphate (CAS No: 4259-15-8) and Zinc bis (O, O-bis (1, 3-dimethylbutyl) dithiophosphate(CAS No: 2215–35–2),it can be concluded thatchemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) is able to cause severe eye irritation and considered as irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Category 1”.

 

Justification for classification or non-classification

The skin and eye irritation potential of test chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) and its closest read across substance with log kow as the primary descriptorwere observed in various studies. The results obtained from these studies indicate that the chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) is unlikely to cause skin irritation but can cause severe eye damage. Hence 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No: 63589-47-9) can be classified under the category “Not Classified” for skin and “Category 1” for eye as per CLP.