2-hydroxyethyl [(1R,2S,5R)-2-isopropyl-5-methyl-cyclohexyl] carbonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-05-10 to 1994-05-12

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

(1984)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

signed 1994-03-16

Analytical monitoring:

yes

Details on sampling:

- Sampling method: Samples were taken of fresh and the 48-h old media at test end

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct dispersion in water
- Controls: yes, one control

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna Straus
- Source: laboratory culture
- Age of parental stock: gravid adults
- Feeding during test: none

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Adults were isolated 24 hours prior to test initiation and the overnight produced young daphnids were used for testing.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

270 mg/L as CaCO3

Test temperature:

21.0 °C

pH:

7.6 - 7.7

Dissolved oxygen:

at test start 8.4 mg/L
at test end 7.9 - 8.0 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0 (control), 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56, and 100 mg test item/L
For measured concentrations please refer to "any other information on results incl. tables", since not all nominal concentrations were analytically verified at 0 hours.

Details on test conditions:

TEST SYSTEM
- Test vessel:glass jars containing 250 mL test solution
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 25 mL per organism

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (ISO) according to OECD 202
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: only if necessary to 7.8 ± 0.2
- Photoperiod: 16 h light and 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobilisation


RANGE-FINDING STUDY
- Test concentrations: 0.1, 1.0, 10, and 100 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

42 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 %-CI: 36 - 51 mg/L

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

not applicable

Reported statistics and error estimates:

Analysis of the immobility data by the moving average method of Thompson (1947).

Cumulative Immobilisation

Nominal Concentration (mg/L)	Cumulative immobilised Daphnia (initial population: 10 per replicate)
	24 hours				48 hours
	R1	R2	Total	%	R1	R2	Total	%
Control	0	0	0	0	0	0	0	0
1.0	0	0	0	0	0	0	0	0
1.8	0	0	0	0	0	0	0	0
3.2	0	0	0	0	0	0	0	0
5.6	0	0	0	0	0	0	0	0
10	0	0	0	0	0	0	0	0
18	0	0	0	0	0	0	0	0
32	0	0	0	0	4	3	7	35
56	4	3	7	35	7	6	13	65
100	10	10	20	100	10	10	20	100

R₁and R₂= Replicates 1 and 2

Analytical results for the test item

Samples	Nominal concentration (mg/L)	Concentration found (mg/L)	Concentration expressed as % of nominal
0 hours	Control 1.0 3.2 10 32 100	<L.O.D. 0.938 2.88 8.94 28.3 87.5	- 93.8 90.0 89.4 88.6 87.5
48 hours	Control 1.0 1.8 3.2 5.6 10 18 32 56 100	<L.O.D. 1.01 1.73 3.19 4.72 9.83 16.5 25.3 46.1 84.9	- 100.6 95.6 99.6 84.3 98.3 91.6 79.0 82.3 84.9

L.O.D. = Limit of detection

Validity criteria fulfilled:

yes

Conclusions:

The test item was found to immobilise Daphnia magna at concentrations higher than 18 mg/L after 48 hours. Based on the nominal test item concentrations, the 48-hour EC50 for Daphnia magna amounts to 42 mg/L (95 %-CI: 36 - 51 mg/L).

Executive summary:

The toxic effect of the test item to Daphnia magna was examined according to OECD 202 (1984) under static conditions over a period of 48 hours. For the determination of the test concentrations, a range-finding test was performed. In the definitive test, each test concentration and the control was tested in duplicate with ten daphnids each. Effect concentrations were calculated based on nominal test concentration since analytical recoveries were in the range of 80 to 120 % throughout the test. After 48 hours of exposure the test item was found to immobilise Daphnia magna at concentrations above 18 mg/L (NOEC). The corresponding 48-hour EC50 value was estimated with 42 mg/L (95 %-CI: 36 - 51 mg/L).

Description of key information

The test item was found to immobilise Daphnia magna at concentrations above 18 mg/L (NOEC). The 48-hour EC50 value was estimated with 42 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

42 mg/L

Additional information

The toxic effect of the test item to Daphnia magna was examined according to OECD 202 (1984) under static conditions over a period of 48 hours. For the determination of the test concentrations, a range-finding test was performed. In the definitive test, each test concentration and the control was tested in duplicate with ten daphnids each. Effect concentrations were calculated based on nominal test concentration since analytical recoveries were in the range of 80 to 120 % throughout the test. After 48 hours of exposure the test item was found to immobilise Daphnia magna at concentrations above 18 mg/L (NOEC). The corresponding 48-hour EC50 value was estimated with 42 mg/L (95 %-CI: 36 - 51 mg/L).