N,N-bis(2-hydroxyethyl)-3-[(C16-18)alkoxy]-1-propanamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2nd September - 15th October 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Stored at room temperature in vented hood.

Analytical monitoring:

yes

Details on sampling:

Samples were removed from each replicate chamber on Day 0 (1 hour and 15 minutes after the addition of test material), Day 2 and Day 4 (or at termination of replicate) for Dissolved Organic Carbon (DOC) analysis.

Vehicle:

no

Details on test solutions:

The nominal treatment concentrations for the test were 4.Og/L, 1.8g/L, 0.81g/L., 0.36g/L, O.16g/L and a laboratory dilution water control. Each treatment was prepared by adding the appropriate amount of test material to each replicate test tank. After allowing the test material to circulate through the dispersion system, samples were removed from each replicate for Dissolved Organic Carbon (DOC) analysis.

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

The sheepshead minnow, Cyprinodon variegatus has historically been used in safety evaluation and is a common test species for marine and estuarine toxicity studies.

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Observations for mortality, abnormal behavior and appearance of the fish were performed on all replicate chambers at 24, 48, 72 and 96 hours (± I hr).

Test temperature:

Temperature measurements were performed daily on each test chamber. Mean test temperature: 21.5°C (sd = 0.4), continuously monitored.

pH:

pH measurements were performed daily in each cahmber. pH range: 8.1 - 8.5

Dissolved oxygen:

Dissolved oxygen measurements were performed daily in each chamber. Range: 6.6 - 7.1 mg/L

Salinity:

Salinity was measured at 25 ppt.

Nominal and measured concentrations:

Nominal concentrations were: 0.16, 0.36, 0.81, 1.8 and 4 g/L

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.69 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The 24, 72 and 96 hour Lethal Concentration (LC) value with 95% confidence intervals was determined using the Spearman-Karber Method3. The 48 hour LC50 value with 99% confidence intervals was determined using the Binomial Method4. The Lethal Concentration is the calculated nominal test material concentration that is lethal to 50% of the test organisms within a specified exposure period. The LC50 values with their confidence intervals are as follows:
Exposure Period LC50 Confidence Intervals
(hours) (g/L) (g/L)
24 2.58 2.38 - 2.79
48 1.30 0.81 - 1.80
72 1.07 0.94 - 1.22
96 0.69 0.58 - 0.81
The maximum concentration which caused no mortality was 0.36g/L. The minimum concentration which caused 100% mortality was 1.8g/L.
The test material was observed floating at the surface of the test chambers.

Sublethal observations / clinical signs:

An initial rangefinding test was performed to determine the definitive test concentrations. The nominal concentrations tested were 5.0 g/L, 1.0 g/L, 0.5 g/L, 0.05 g/L and 0.0l g/L. A laboratory dilution water control was also tested. One replicate was prepared for each concentration. The test was run in a dispersion system. Five organisms were exposed to each concentration for 48 hours. Total mortality was observed in the 5.0 g/L concentration after 24 hours and 40% mortality was observed in the 1.0 g/L concentration after 48 hours. No mortality or abnormal behavior was observed in the remaining concentrations.

Validity criteria fulfilled:

yes

Conclusions:

The maximum concentration which caused no mortality was 0.36g/L. The minimum concentration which caused 100% mortality was 1.8g/L. The test resulted in a 96 hour LC50 value of 0.69g/L with 95% confidence intervals of 0.58 to 0.81g/L. The Lethal Concentration (LC) is the calculated nominal test material concentration that is lethal to 50% of the test organisms within a specified exposure period.

Executive summary:

A sheepshead minnow (Cyprinodon variegatus) test was performed to evaluate the acute toxicity of the test material.

The test was performed in a dispersion system. The dispersion system consists of 19.0L glass tanks containing 12cm long stainless steel cylinders (7.5cm diameter) in the center which house a stainless steel rod and propeller. The motorized stirrers were set at a speed in which an approximately one inch vortex was formed in each tank.

The nominal treatment concentrations for the test were 4.0 g/L, 1.8 g/L, 0.81 g/L., 0.36 g/L, 0.16 g/L and a laboratory dilution water control. Each treatment was prepared by adding the appropriate amount of test material to each replicate test tank. After allowing the test material to circulate through the dispersion system, samples were removed from each replicate for Dissolved Organic Carbon (DOC) analysis. The sheepshead minnows were exposed for a 96 hour period.

The test resulted in a 96 hour LC50 value of 0.69g/L with 95% confidence intervals of 0.58 to 0.81g/L. The Lethal Concentration (LC) is the calculated nominal test material concentration that is lethal to 50% of the test organisms within a specified exposure period.

Description of key information

A sheepshead minnow (Cyprinodon variegatus) test was performed to evaluate the acute toxicity of the test material.

The test was performed in a dispersion system. The dispersion system consists of 19.0L glass tanks containing 12cm long stainless steel cylinders (7.5cm diameter) in the center which house a stainless steel rod and propeller. The motorized stirrers were set at a speed in which an approximately one inch vortex was formed in each tank.

The nominal treatment concentrations for the test were 4.0 g/L, 1.8 g/L, 0.81 g/L., 0.36 g/L, 0.16 g/L and a laboratory dilution water control. Each treatment was prepared by adding the appropriate amount of test material to each replicate test tank. After allowing the test material to circulate through the dispersion system, samples were removed from each replicate for Dissolved Organic Carbon (DOC) analysis. The sheepshead minnows were exposed for a 96 hour period.

The test resulted in a 96 hour LC50 value of 0.69 g/L with 95% confidence intervals of 0.58 to 0.81 g/L. The Lethal Concentration (LC) is the calculated nominal test material concentration that is lethal to 50% of the test organisms within a specified exposure period.

Key value for chemical safety assessment

Marine water fish

Marine water fish

Effect concentration:

0.69 g/L

Additional information

According to the Regulation (EC) No 1272/2008 the classification criteria are not met.