Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 240-212-2 | CAS number: 16068-37-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Version / remarks:
- (March 1991)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on test solutions:
- Test media were prepared by vigorous stirring overnight plus two hours separation.
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- TEST ORGANISM
- Common name: Pseudomonas putida
- Strain: DSM 50026 (lyophilisate, obtained March 1993).
- Source: MIGULA stock culture
Stock cultures of Pseudomonas putida were streaked on nutrative media for stock cultures, incubated overnight at 25 ˚C and kept at 4 ˚C. Bacteria were transferred to fresh media on a monthly basis. These cultures were used as original cultures in the present test. Prior to the main test, the bacteria strain was adapted to liquid medium by cultivating in nutritive solution for pre-cultures. After incubating overnight at 21 ˚C, the turbidity of the bacterial suspension was measured and adjusted to TE/F 50 by addition of medium for pre-culture. This suspension was used as inoculum for the test pre-culture.
A test pre-culture with an initial turbidity of ca. TE/F 5 was prepared and incubated for 7h at 20 - 22 ˚C. The turbidity was measured and adjusted to ca. TE/F 10 by addition of test medium. This suspension was used as inoculum for the test culture. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Test temperature:
- 20 - 22 ˚C
- pH:
- not stated.
- Nominal and measured concentrations:
- 700, 2850, 4250, 5650, 8000 mg/l (nominal) plus control (0 mg/l)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml Erlenmeyer flasks
- No. of vessels per concentration (replicates): performed in triplicate
- No. of vessels per control (replicates): performed in triplicate
- No. of vessels per vehicle control (replicates): performed in triplicate
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Purified water was used for all solutions and media
OTHER TEST CONDITIONS
- Photoperiod: none
- Light intensity: dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Turbidity
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10 g/l, 1.0 g/l (plus Tween 80) and 100 mg/l - Reference substance (positive control):
- no
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 8 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: cell multiplication
- Results with reference substance (positive control):
- Within the last representative reference test (21-23 July 1993) with 3,5-dichlorophenol tested under the same conditions gave EC50 and EC10 values of 17.3 mg/l and 6.5 mg/l respectively. This EC50 is in keeping with the interlaboratory comparison results of 21.4 ± 4.91 mg/l though the EC10 is lower than the interlaboratory comparison of 13.7 ± 4.36 mg/l.
- Validity criteria fulfilled:
- yes
- Remarks:
- mean biomass in controls increased by more than 100-fold. A recent reference test using 3,5-dichlorophenol tested under the same conditions gave typical inhibitory EC50 results.
- Conclusions:
- A NOEC of ≥8000 mg/l (nominal, highest concentration tested) was obtained for inhibition of cell multiplication in a P. putida cell multiplication inhibition test according to Guideline DIN 38412 and in compliance with GLP. The result is considered reliable.
Reference
Description of key information
Short-term toxicity to microorganisms: 16 hour EC50 >8000 mg/L and NOEC ≥8000 mg/L (nominal) (highest concentration tested) (DIN 38412 part 8), based on inhibition of cell multiplication in P. putida.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 8 000 mg/L
- EC10 or NOEC for microorganisms:
- 8 000 mg/L
Additional information
A 16-hour EC50 value of >8000 mg/L and NOEC of ≥8000 mg/L (nominal) (highest concentration tested) have been determined for the effects of 4,4,7,7-tetraethoxy-3,8-dioxa-4,7-disiladecane (CAS 16068-37-4) on cell multiplication of Pseudomonas putida. In view of the test media preparation method it is likely that the test organisms were exposed to a mixture of the parent substance and its hydrolysis products, which could have included some cross-linked products.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
