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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Biodegradation (28d) = 68.38 %; not readily biodegradable.

Key value for chemical safety assessment

Additional information

The ready biodegradability of the substance was assessed according to ASTM Guideline E 1720 -01, which is similar to OECD Guideline 301B. The test substance in a mineral medium was inoculated with the domestic activated sludge for 28 days under aerobic conditions. During this period the biodegradation of the test substance was determined based on CO2 production. CO2 evolution was determined on the days 0, 3, 7, 10, 14, 17, 20, 24, 28. Concurrent blank (medium and inocolumn) and reference (dextrose, medium and inocolumn) run in parallel.

The substance recorded a biodegradability of 68.38 % on day 28. According to the ASTM E1720: the method requirement for Readily Biodegradability is a minimum of 60 % organic carbon conversion measured via CO2 evolution achieved in a 10 -day window after passing the 10 % Biodegradation threshold. The method requirement for Ultimately Biodegradability is a minimum of 60 % organic carbon conversion measured via carbon dioxide evolution achieved within the 28-day testing period. Based on the afore mentioned requirements, the test substance can be considered as non-readily biodegradable since it failed to meet the 10 -day window criterion, but it can be considered as ultimately biodegradable.