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Short-term toxicity to fish:

A static renewal fathead minnow (Pimephales promelas) chronic toxicity test (7-days) was performed with Iso bornyl cyclo hexanol according to EPA/600/4-90/027 and ASTM's Methods E729, 1997, Volume 11.05.

- LC50: 1.36 mg/L

-  NOEC: 0.34 mg/L (based on growth)

-  LOEC: 1.36 mg/L (based on survival)

With a LC50-7days: 1.36 mg/L, isobornylcyclohexanol is toxic for fish.

Short-term toxicity to aquatic invertebrates:

In a 48–hr-acute toxicity study, daphnids (Daphnia similis) were exposed to isobornylcyclohexanol at 1, 10 and 100 mg/L (nominal concentrations) under static conditions according to the similar OECD guideline 202. 

The 48– hour EC50 was between 1 and 10 mg/L. 

Based on the results of isobornylcyclohexanol is toxic to aquatic invertebrates.

Toxicity to aquatic algae:

In a 72 -hr-growth inhibition study, algae (Pseudokirchneriella subcapitata) were exposed to Rhodiantal original IBCH at 100 mg/L (nominal loading rate WAF) according to the OECD guideline 201.

The 72-hour EL50 of RHODIANTAL ORIGINAL IBCH to Pseudokirchneriella subcapitata has determined to be > 100 mg/L (nominal loading rate WAF) based on the growth rate; revealing it is not harmful for the algae.

The 72h No-Observed Effect Loading Rate (NOELR):               100 mg/L loading rate WAF

The 72h Lowest Observed Effect Loading Rate (LOELR):       > 100 mg/L loading rate WAF

Long-term toxicity to aquatic invertebrates :

A static renewal Ceriodaphnia dubia chronic toxicity test (7-days) was performed with Iso bornyl cyclo hexanol according to EPA/600/4-90/027 and ASTM's Methods E729, 1997, Volume 11.05.

- LC50: 1.93 mg/L

-   NOEC: 0.68 mg/L (based on reproduction)

-   LOEC: 1.36 mg/L (based on reproduction)

With a NOEC-7days: 0.68 mg/L, isobornylcyclohexanol is toxic to aquatic invertebrates with long lasting effects.

Toxicity to microorganisms:

The inhibitory effect of Rhodiantal original IBCH on the respiration of the activated sludge was investigated in a 3 -hour respiration inhibition test according to OECD guideline for testing of chemicals n°209 (2010).

EC50 : > 1000 mg/L (total respiration)

EC10 : 318 mg/L

Based on the results, Rhodiantal original IBCH has no effect on the respiration rate of activated sludge microorganisms.

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