Phosphoric acid, C12-18-alkyl esters, potassium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Remarks:

Data are included only to justify the read-across approach of the dossier

Adequacy of study:

key study

Study period:

2012-02-14 to 2012-02-17

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Silastol H 200 TS

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

As solubility lies below 100 mg/L, the water-accommodated fraction was prepared for the test. This was done by weigh ing the nominal load,
adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was unfiltrated used as test solution.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna / STRAUS
- Strain: Berlin
- Source: Umweltbundesamt Berlin/ Daphnia magna is bred in the LAUS GmbH throughout the year
- Age at study initiation (mean and range, SD): 0 - 24 hours
- Weight at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): no data
- Valve height at study initiation, for shell deposition study (mean and range, SD): no data
- Peripheral shell growth removed prior to test initiation: no data
- Method of breeding: Daphnia magna is bred in the LAUS GmbH throughout the year. The husbandry is performed similar to the method described in EN ISO 6341
- Feeding during test none


ACCLIMATION
- Acclimation conditions (same as test or not): no data
- Type and amount of food: unicellular green algae (Desmodesmus subspicatus)
- Feeding frequency: twice a week

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

2.502 mmol/L

Test temperature:

Temperature range was 18.0 - 19.7°C.

pH:

The pH values in the test media and the control ranged from 7.6 to 7.7.

Dissolved oxygen:

The concentration of dissolved oxygen stayed above 9.4 mg/L throughout the test.

Nominal and measured concentrations:

100 mg/L nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers, nominal volume 50 mL, tall shape
- Material, size, headspace, fill volume: 50 mL
- Aeration: no

- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS no data

OTHER TEST CONDITIONS
- Adjustment of pH: After preparation, the dilution water is aerated, and the pH is adjusted to 7.8 ± 0.2.
- Photoperiod: non


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

The immobilised daphnia were counted. Daphnia are considered immobilised if they perform no movements or are only able to move their antennae
when the beaker is gently agitated. Daphnia which are trapped at the surface of the test solution are also considered immobilised.

Reference substance (positive control):

yes

Remarks:

Potass ium dichromate K2Cr207 (CAS No. 7778-50-9)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: water-accommodated fraction (WAF)

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: water-accommodated fraction (WAF)

Basis for effect:

mobility

Details on results:

None of the daphnia died or showed any signs of abnormal behaviour throughout the test.

Results with reference substance (positive control):

NOEC 24 h = 0.625 mg/L
24h EC50 = 1.7 mg/L (1.2 - 2.5 mg/L)
24h EC100 = 3 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The EC50 was determined to be > 100 mg/L Silastol H 200 TS (WAF based on nominal concentration).
The NOEC was determined to be ≥ 100 mg/L Silastol H 200 TS (WAF based on nominal concentration).

Executive summary:

All validity criteria were met. For the estimation of the EC50 of the positive control, the fit showed sufficient statistical corre spondence of the data with the dose-response-equation. The 24h -EC50 of potassium dichromate was determined as 1.7 mg/L, lying within the demanded range of 0.6 - 1.7 mg/L. In the main study, the treatment showed no toxicity. None of the animals were immobilised in the control.

Because of the poor solubility in water, the analytical determin ation s of the test item showed weak correlation between nominal and measured concentration s. The measured DOC values in the test solution and in the control were below the limit of quantification. Therefore, the determination of the results was based on the nomin al concentrations. The result of the test can be considered valid.

None of the daphnia died or showed any signs of abnormal behaviour throughout the test.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016-10-10 to 2017-02-06

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Remarks:

laboratory is accredited for this test method according to ISO 17025

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: STRAUS
- Source: Umweltbundesamt Berlin
- Age of parental stock (mean and range, SD): no information
- Feeding during test: no


ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food: green algae
- Feeding frequency: daily
- Health during acclimation (any mortality observed):

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

18-22 °C

pH:

7.4

Nominal and measured concentrations:

nominal concnetrations:
Test 1: 1.0, 2.0, 4.0, 7.0, 10, 22, 48, 100, 200 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: test tube
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8
- Light intensity: 500 - 600 Lux


VEHICLE CONTROL PERFORMED: no

Reference substance (positive control):

yes

Remarks:

Test performed on 2017-01-10

Key result

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid: yes
- Relevant effect levels: EC50 (24h): 1.81 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 of Phosphoric acid, C12-18 alkyl esters, potassium salts was > 200 mg/L (nominal).
 

Executive summary:

The 48-hr-acute toxicity of Phosphoric acid, C12-18 alkyl esters, potassium salts to Daphnia magna was studied under static conditions according to OECD Guideline 202. Daphnids were exposed to control and test chemical at nominal concentrations of 1.0, 2.0, 4.0, 7.0, 10, 22, 48, 100, 200 mg/L a.i./L in fresh water for 48 h.

 

The 48-hour EC50 was > 200 mg/L nominal

 

This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

 

Results Synopsis

Test Organism Age: max. 24 h old daphnids

Test Type: Static

 

48h-EC50: > 200 mg/L nominal

Endpoint(s) Effected:  mobility

Description of key information

The 48-hour EC50 of 100 mg/L was used for chemical safety assessment of Phosphoric acid, C12-18 alkyl esters, potassium salts.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The 48-hr-acute toxicity of Phosphoric acid, C12-18 alkyl esters, potassium salts to Daphnia magna was studied under static conditions according to OECD Guideline 202.

Daphnids were exposed to control and test chemical at nominal concentrations of 1.0, 2.0, 4.0, 7.0, 10, 22, 48, 100, 200 mg/L a.i./L in fresh water for 48 h.

The 48-hour EC50 was > 200 mg/L nominal (based on mobility).

 

Similar results were obtained with the read-across substance 1-Octadecanol, phosphate, potassium salt. In a study concocted according to OECD guideline 202, the 48h-EC50 was determined to be > 100 mg/L

(WAF based on nominal concentration). The NOEC was determined to be ≥ 100 mg/L (WAF based on nominal concentration).