Vinylcyclohexane

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

July - September 1965

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

publication

Title:

The toxicology of vinylcyclohexane

Author:

Savchenkov M.F.

Year:

1965

Bibliographic source:

Gigiena i Sanitariya 30 Vol. 7, 32-37

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The experiments were performed on white mice and albino rats, some being healthy adult and young animals and some weakened by avitaminosis and insufficient feeding. The animals were kept in chambers that were exposed to vapours of the substance whose concentration was predetermined by a method developed by a worker at the Dushutin Institute.

GLP compliance:

not specified

Test type:

traditional method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Vinylcyclohexane

EC Number:

211-779-3

EC Name:

Vinylcyclohexane

Cas Number:

695-12-5

Molecular formula:

C8H14

IUPAC Name:

ethenylcyclohexane

Test animals

Species:

other: white mice and albino rats

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

not specified

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on study design:

The experiments were performed on white mice and albino rats, some being healthy adult and young animals and some weakened by avitaminosis and insufficient feeding. The concentration of the substance in the chamber was determined by amethod developed by a worker at the Dushutin Institute which is based on the color resulting from the reaction of VCH with concentrated sulfuric acid.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

13.7 mg/L air

Based on:

test mat.

Remarks on result:

other: healthy mice

Effect levels 2

Sex:

male/female

Dose descriptor:

LC50

Effect level:

20.5 mg/L air

Based on:

test mat.

Remarks on result:

other: healthy rats

Mortality:

Mortality occurred and some animals died during the spasmodic attacks.

Clinical signs:

other: - The animals exposed to high concentrations of these vapours immediately showed signs of excitation, increased breathing and staggering gait. They fell, rose again, were suddenly seized by tonic spasms of extreme intensity, fell on their sides and develo

Body weight:

Not specified

Gross pathology:

Not specified

Other findings:

- The substance possessed a narrow toxic range and was more highly toxic for the young and weakened animals
- There were no significant differences in the sensitivity of males and females
- the absolute lethal concentration was 15 mg/L and partially lethal concentration was 12.5 mg/L in healthy mice
- In case of weakened mice the LC50 was 10.6 mg/L
- The LC50 for young rats (60 - 80 g bw) was 17 mg/L
- Animals that had survived the acute poisoning (exposed to LD50) were again exposed to the test item vapour concentration of 15 mg/L for 2 hours the following day. Not a single experimental animal died and none even fell on its side

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

Based on the results the LC50 for rats was estabilshed to be 20.5 mg/mL and 13.7 mg/L for mice.

Executive summary:

Based on the results, the LC50 for rats was estabilshed to be 20.5 mg/mL and 13.7 mg/L for mice.