Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 908-081-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 January 2017 - 02 February 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch/Lot Number: 150205
Description: Blue powder
Purity1: As a UVCB, purity is considered as 100%
Expiry date: 05 February 2019
Storage condition: Controlled room temperature (15-25°C, below 70 RH%) - Analytical monitoring:
- yes
- Details on sampling:
- A saturated test item solution (nominal loading rate of 100 mg/L) was prepared by dispersing/dissolving the amount of test item into the test medium (ISO Medium) two days before the start of the experiment. This solution was shaken for about 24 hours at approximately 30°C and then equilibrated for about 24 hours at approximately 20°C. The non-dissolved test material was removed by filtration through a fine (0.22 μm) filter to give the nominal loading rate of 100 mg/L.
The sample was homogenized by shaking manually. An aliquot of 2 ml of the sample containing the test item was diluted to 10 mL with nitric acid (1:10). Tha same procedure was used for each sample. - Vehicle:
- no
- Details on test solutions:
- A saturated test item solution (nominal loading rate of 100 mg/L) was prepared by dispersing/dissolving the amount of test item into the test medium (ISO Medium) two days before the start of the experiment. This solution was shaken for about 24 hours at approximately 30°C and then equilibrated for about 24 hours at approximately 20°C. The non-dissolved test material was removed by filtration through a fine (0.22 μm) filter to give the nominal loading rate of 100 mg/L.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Feeding during test: no
ACCLIMATION
- Acclimation period: none - Test type:
- semi-static
- Water media type:
- other: Reconstituted water (ISO medium, according to OECD 202)
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 248 mg/L (as CaCO3)
- Test temperature:
- test vessels: 20.2 – 20.5°C
climate chamber: 19.8 and 20.7°C - pH:
- 7.25 – 7.49
- Dissolved oxygen:
- 8.2 – 8.9 mg/L
- Nominal and measured concentrations:
- 100 mg/L nominal loading rate WAF
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass beaker
- Renewal rate of test solution (frequency/flow rate): 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16-hour light and 8-hour dark cycle
RANGE-FINDING STUDY
- Test concentrations: 100, 10 and 1 mg/L nominal loading rates WAFs
- Results used to determine the conditions for the definitive study: toxic response was not observed during the preliminary concentration rangefinding test, a Limit Test was carried out using only one test concentration at 100 mg/L nominal loading rate WAF and one control group in a semi-static system. - Reference substance (positive control):
- yes
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOELR
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Details on results:
- There was no immobilization in the control group and the dissolved oxygen concentration at the end of the test in control and test vessels was more than 3 mg/L.
- Results with reference substance (positive control):
- The 24-hour EC50 values of Potassium dichromate were within the range 0.6 – 2.1 mg/L (ISO Guideline No. 6341
The 24h EC50: 0.93 mg/L, (95 % confidence limits: 0.86 – 0.99 mg/L).
In addition to immobility, no abnormal behaviour or appearance of test animals was detected. - Reported statistics and error estimates:
- No statistical analysis was performed because of the lack of toxic effects.
The EL50, NOELR, LOELR and EL100 values were determined directly from the raw data. - Validity criteria fulfilled:
- yes
- Conclusions:
- Acute toxicity of the test item was assessed with Acute immobilisation test on Daphnia magna, over an exposure period of 48 hours in a semi-static system.
All validity criteria were met during this study.
Under the conditions of this Daphnia magna acute immobilisation study the observed endpoints for the effect of B440 were the followings:
The 24h and 48h EL50 value: > 100 mg/L nominal loading rate WAF
The 48h EL100 value: > 100 mg/L nominal loading rate WAF
The 48h No-Observed Effect Loading Rate (NOELR): 100 mg/L nominal loading rate WAF
The 48h Lowest Observed Effect Loading Rate (LOELR): > 100 mg/L nominal loading rate WAF
Reference
Description of key information
The 24h and 48h EL50 value: > 100 mg/L nominal loading rate WAF
The 48h EL100 value: > 100 mg/L nominal loading rate WAF
The 48h No-Observed Effect Loading Rate (NOELR): 100 mg/L nominal loading rate WAF
The 48h Lowest Observed Effect Loading Rate (LOELR): > 100 mg/L nominal loading rate WAF
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
