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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
20000.6 mg of the test item was added to 1 000 mL reconstituted test water (dilution water) and stirred on the day of the test start. The test solutions were made by dilution of the stock solution (approx. 20 000 mg/L) with reconstituted test water.
Test organisms (species):
Daphnia magna
Details on test organisms:
Test organisms were newly hatched, between 6 and 24 hours old. Animals originated from Toxicon's continuous culture of Daphnia magna.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
250±25 mg/L calcium carbonate
Test temperature:
21±1°C
pH:
7.8±0.2
Dissolved oxygen:
The report mentions that that dissolved oxygen was measured in pooled vessels at th end of the exposure period and states that the validity criterion for dissolved oxygen to remain above 3 mg/L during the test was met but the measured values are not reported.
Salinity:
Not applicable
Conductivity:
Not applicable
Nominal and measured concentrations:
Nominal exposure concentrations were 0 (control), 1250, 2500, 5000, 10000 and 20000 mg/L.
Details on test conditions:
Reconstituted test water, as specified in OECD TG no. 202, was used as dilution water (specified below). In the controls, only reconstituted test water was used. The animals were incubated in glass Petri dishes (50 mL), with 25 mL solution. Four replicates, with five animals in each replicate, were used for both the test concentrations and for the control. The dishes were incubated in a thermo constant room at a temperature of 21±1° C and subdued light with the light rhythm of 16 hours light: 8 hours darkness. Before the experiment start, pH and oxygen saturation was measured in all the solutions. The numbers of immobilised animals were observed at 24 and 48 hours in all the test dishes. At the end of the experiment pH and oxygen saturation were measured in all the test groups (4 pooled replicates/group).
Reference substance (positive control):
yes
Remarks:
Reference material not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7 060 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
2 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Immobility results are presented in the report in the form of a graph. After 24 hours immobility was 0% in the control, 250 and 2500 mg/L treatments, 10% at 5000 mg/L, 85% at 10000 mg/L and 100% at 20000 mg/L. After 48 hours immobility was 0% in the control, 250 and 2500 mg/L treatments, 15% at 5000 mg/L and 100% at 10000 and 20000 mg/L.
Results with reference substance (positive control):
The report states the following:
A qualification test on the Daphnia magna culture was made on the 15th of May 2008 to assure the quality of the test animals. EC50 was 1,3 mg/l, which confirmed that the culture was suitable as a test organism according to OECD TG no. 202.
Reported statistics and error estimates:
The EC50-values was calculated by means of graphic interpolation. Probit-analysis was not carried out due to lack of data. The NOEC-value is defined as the highest tested concentration where the percent immobilisation is ≤10%.
Validity criteria fulfilled:
yes
Conclusions:
Based on nominal exposure concentrations the 48 hour EC50 of Reaction mass of 2,2-bis(formyloxymethyl)propane-1,3-diyl diformate and formic acid / Formic acid tech 50% / 902-307-3 to Daphnia magna was determined by graphical interpolation to be 7060 mg/L.
Executive summary:

The acute toxicity of Reaction mass of 2,2-bis(formyloxymethyl)propane-1,3-diyl diformate and formic acid / Formic acid tech 50% / 902-307-3 to Daphnia magna was determined. The study was carried out according to OECD test guideline. In this study Daphnia magna were exposed to the test material over a test period of 48 hours under static conditions. Nominal exposure concentrations were 0 (control), 1250, 2500, 5000, 10000 and 20000 mg/L.  The results of the study were based on the nominal concentrations.

 

Based on nominal exposure concentrations the 48 hour EC50 of Reaction mass of 2,2-bis(formyloxymethyl)propane-1,3-diyl diformate and formic acid / Formic acid tech 50% / 902-307-3 to Daphnia magna was determined by graphical interpolation to be 7060 mg/L.

Description of key information

In an OECD 202 test the 48-hour EC50 was determined to be 7060 mg/L and the NOEC 2500 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
7 060 mg/L
EC50/LC50 for marine water invertebrates:
2 500 mg/L

Additional information

A semi-static GLP study to OECD 202 was carried out to determine the toxicity to aquatic invertebrates (Daphnia). The test substance demonstrated low toxicity to Daphnia in this acute study. The nominal 48 hour EC50 was found to be 7060 mg/L with a NOEC of 2500 mg/L. The test substance should be considered non toxic to Daphnia.