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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987

Materials and methods

Test guidelineopen allclose all
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 870.1300 (Acute inhalation toxicity)
GLP compliance:
yes
Test type:
traditional method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl 3-mercaptopropionate
EC Number:
220-912-4
EC Name:
Methyl 3-mercaptopropionate
Cas Number:
2935-90-2
Molecular formula:
C4H8O2S
IUPAC Name:
methyl 3-sulfanylpropanoate
Details on test material:
IUCLID4 Test substance: as prescribed by 1.1 - 1.4

Test animals

Species:
rat
Strain:
other: Crl:CD(SD)BR
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
head only
Vehicle:
air
Remark on MMAD/GSD:
n.a. for vapour
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
276, 350, 456, 493, and 529 ppm (mean measured), equivalent to 1.35, 1.72, 2.24, 2.42, and 2.60 mg/L, respectively
No. of animals per sex per dose:
60
Control animals:
no

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LC50
Effect level:
430 ppm
Exp. duration:
4 h
Key result
Sex:
male/female
Dose descriptor:
LC50
Effect level:
2.11 mg/L air (analytical)
Based on:
test mat.
95% CL:
>= 1.89 - <= 2.25
Exp. duration:
4 h
Mortality:
There were 25 deaths (12 males; 13 females) during the exposure period. There were no deaths during the 14-day observation period. The incidence of these deaths was exposure-level related, but there was no evident difference between the sexes. All animals died at the highest level, as shown below:

Measured Mortality Ratio
Conc.(ppm) Males:Females
Control 0/5:0/5
276 0/5:0/5
350 0/5:1/5
456 2/5:3/5
493 5/5:4/5
529 5/5:5/5
Clinical signs:
other: Chromodacryorrhoea was the main sign observed during and immediately following the exposure period in both test and control animals; it was considered to be attributable to the restraint procedure used in exposure. The few clinical signs observed in ani
Body weight:
A small temporary body weight loss in control and test animals was considered to be attributable to the procedure used for animal restraint.
Gross pathology:
There were no gross or histopathological evidence of target organ toxicity to account for the deaths.
The pathology of control and treated rats surviving to termination was unremarkable at necropsy or had minor findings such as uterine distension. Histopathology findings were also minor such as leucocyte foci in lung and liver and were consistent with the expected pattern of background pathology in rats of
this strain and age. There were no gross or histopathological findings suggestive of irreversible or delayed toxicity due to test article administration.

Any other information on results incl. tables

The exposure chamber temperature recorded for the control and treated groups ranged between 21 and 22 °C, and the chamber humidity was between 49 and 82%. The chamber air flow rates were in the range of 5.2 and 12.0 L/min. The oxygen concentration ranged between 20 and 21% for the control and treated animals throughout the study. The mean measured concentrations of MMP were 276, 350, 456, 493, and 529 ppm for the nominal concentrations of 396, 437, 682, 595, and 815 ppm, respectively.

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria
Conclusions:
The 4-h Inhalation LC50 of MMP as vapour is 430 ppm (2.11 mg/L).

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