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Diss Factsheets

Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983

Materials and methods

Test guidelineopen allclose all
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 870.1200 (Acute Dermal Toxicity)
GLP compliance:
yes
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl 3-mercaptopropionate
EC Number:
220-912-4
EC Name:
Methyl 3-mercaptopropionate
Cas Number:
2935-90-2
Molecular formula:
C4H8O2S
IUPAC Name:
methyl 3-sulfanylpropanoate
Details on test material:
IUCLID4 Test substance: as prescribed by 1.1 - 1.4

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male/female

Administration / exposure

Type of coverage:
not specified
Vehicle:
unchanged (no vehicle)
Duration of exposure:
24 h
Doses:
1000, 1500, 2000, and 2500 mg/kg bw
No. of animals per sex per dose:
3
Control animals:
not required

Results and discussion

Effect levelsopen allclose all
Sex:
female
Dose descriptor:
LD50
Effect level:
1 892 mg/kg bw
Based on:
test mat.
95% CL:
>= 1 384 - <= 2 584
Sex:
male
Dose descriptor:
LD50
Effect level:
1 930 mg/kg bw
Based on:
test mat.
95% CL:
>= 1 223 - <= 3 049
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 905 mg/kg bw
Based on:
test mat.
95% CL:
>= 1 462 - <= 2 479
Mortality:
1000 mg/kg bw: all animals survived
1500 mg/kg bw: 2 m / 1 f died
2000 mg/kg bw: 1 m / 2 f died
2500 mg/kg bw: 2 m / 2 f died
Clinical signs:
other: All animals dosed 1000 mg/kg bw of body weight appeared normal during the study with exception of slight depression in one male at 1 hour and one female at 4 hours. Effects of toxicity in the remaining animals were noted by 1 hour post dosing and includ
Gross pathology:
Gross pathological findings among rabbits found dead were limited to dark red lungs in one female dosed at 2000 mg/kg bw of body weight. There were no observable gross pathological findings related to test material administration in any of the rabbits surviving to termination.
Other findings:
Dermal Irritation:
With one exception, dermal irritation scores could not be made in the animals that died during the study because they were found dead prior to Day 1 observation. No dermal irritation was noted on Day 1 in the one female dosed at 2000 mg/kg bw that was found dead on Day 2.
Erythema among survivors ranged from very slight to well-defined. Very slight erythema was noted in one male and one female dosed at 2500 mg/kg bw on Day 1 and decreased in the male to very slight on Day 3, clearing in the female by Day 3 and in the male by Day 7. No edema was noted in one male on Days 7 and 14 and one female on Day 14. Necrosis was noted in one male and female on Day 10 only.

Individual Dermal Irritation Scores:
Animal Erythema Score Edema Score
No. Day Day
1 3 7 10 14 1 3 7 10 14
1000 mg/kg bw
Male 1 0 0 0 0 0 0 0 0 0 0
Male 2 0 0 0 0 0 0 0 0 0 0
Male 3 0 0 0 0 0 0 0 0 0 0
Female 1 0 0 0 0 0 0 0 0 0 0
Female 2 0 0 0 0 0 0 0 0 0 0
Female 3 0 1 0 0 0 0 0 0 0 0

1500 mg/kg bw
Male 1 Found dead Found dead
Male 2 Found dead Found dead
Male 3 1 0 0 0 0 0 0 0 0 0
Female 1 1 0 0 0 0 0 0 0 0
Female 2 1 0 0 0 0 0 0 0 0 0
Female 3 Found dead Found dead

2000 mg/kg bw
Male 1 0 0 0 0 0 0 0 0 0 0
Male 2 Found dead Found dead
Male 3 0 0 0 0 0 0 0 0* 0** 0*
Female 1 Found dead Found dead
Female 2 0 Found dead 0 Found dead
Female 3 0 0 0 0 0 0 0 0 0** 0*

2500 mg/kg bw
Male 1 Found dead Found dead
Male 2 Found dead Found dead
Male 3 2 1 0 0 0 0 0 0 0 0
Female 1 2 0 0 0 0 0 0 0 0
Female 2 Found dead Found dead
Female 3 Found dead Found dead

Where
* = epidermal scaling
** = necrosis

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The dermal LD50 of MMP in the rabbit is 1905 mg/kg bw