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EC number: 215-414-9 | CAS number: 1326-04-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 September 2017 - 01 March 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.20 (Daphnia magna Reproduction Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch: 280PE310516
Purity: UVCB (treat as 100%)
Storage Conditions: Room temperature in the dark - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
range-finding test: 1.0, 10 and 100 mg/L.
Definitive test: 0.056, 0.10, 0.18, 0.32 and 0.56 mg/L
- Sampling method: A sample of each test concentration was taken for chemical analysis on Days 0 and 3 in order to determine the stability of the test item under test conditions
- Sample storage: All samples were stored frozen prior to analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Due to the low aqueous solubility and complex nature of the test item, for the purposes of the study the test medium was prepared as a Water Accommodated Fraction (WAF) of the test item.
- Controls: identical conditions but not exposed to the test item - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: daphnia magna
- Strain/clone: 1st instar
- Justification for species other than prescribed by test guideline: Daphnia magna is a freshwater invertebrate representative of a wide variety of natural habitats, and can therefore be considered as an important non-target organism in freshwater ecosystems.
- Source: in house cultures
- Age of parental stock (mean and range, SD): 24 hours
- Food type: fed daily with a mixture of algal suspension (Desmodesmus subspicatus) and Tetramin® flake food suspension
ACCLIMATION
- Acclimation period: 24 hours
- Acclimation conditions (same as test or not): same
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Remarks on exposure duration:
- not specified
- Post exposure observation period:
- not specified
- Hardness:
- 248 to 290 mg/L as CaCO3
- Test temperature:
- 19°C - 21°C
- pH:
- 7.7 - 8.0
- Dissolved oxygen:
- ≥7.7 mg O2/L
- Salinity:
- not specified
- Conductivity:
- not specified
- Nominal and measured concentrations:
- nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 mL glass vessels
- Type (delete if not applicable): closed
- Aeration: not aerated
- Renewal rate of test solution (frequency/flow rate): three times per week on Days 0, 2, 5, 7, 9, 12, 14, 16 and 19
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): For each test and control group 10 replicate test vessels
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted Water – Elendt M7 Medium
- Culture medium different from test medium: same
- Intervals of water quality measurement: Measurements were made on one replicate for each test concentration, once per week
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness with 20 minute dawn and dusk transition periods for 21 days
- Light intensity: not exceeding 1500 Lux
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1.0, 10 and 100 mg/L., 0.010, 0.10 and 1.0 mg/L.
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOELR
- Effect conc.:
- ca. 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOELR
- Effect conc.:
- ca. 0.18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EL50
- Effect conc.:
- ca. 0.11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- immobilisation
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EL50
- Effect conc.:
- ca. 0.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Behavioural abnormalities:
- Observations on body length and weight: length, no differences statistically
- Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- not specified
- Reported statistics and error estimates:
- 95% confidence limits
- Validity criteria fulfilled:
- yes
- Conclusions:
- Exposure of Daphnia magna to the test item resulted in significant mortalities at the loading rates of 0.18, 0.32 and 0.56 mg/L resulting in 90%, 100% and 100% mortalities by Day 4.
The 21-Day EL50 (immobilization) value, based on nominal loading rates, for the parental daphnia generation (P1) was calculated to be 0.11 mg/L with 95% confidence limits of 0.080 – 0.15 mg/L.
The 21-Day EL50 (reproduction) based on nominal loading rates was 0.13 mg/L with 95% confidence limits of 0.096 – 0.15 mg/L.
The Lowest Observed Effect Loading Rate and the No Observed Effect Loading Rate based on nominal loading rates were 0.18 and 0.10 mg/L respectively.
Reference
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.1 mg/L
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