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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study in compliance with international recognized guidelines
Justification for type of information:
For Weight-of-Evidence Justification please refer to justification documentation in IUCLID Chapter 13.
Cross-reference
Reason / purpose for cross-reference:
assessment report
Remarks:
Justification for Weight-of-Evidence approach

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report date:
2001

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Tin dioxide
EC Number:
242-159-0
EC Name:
Tin dioxide
Cas Number:
18282-10-5
Molecular formula:
O2 Sn
IUPAC Name:
Tin dioxide
Details on test material:
Name of the test item as cited in the report: Tin (IV) oxide extra pure
Appaerance: Whitish powder with a gray tinge.
Purity: 100.3%
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
no data

Test solutions

Vehicle:
yes
Details on test solutions:
The water solubility of tin (IV) oxide is described to be very low and depending on temperature and pressure ( see point 4.8, The solubility of tin from tin dioxide in double distilled water at 20°C is below the limit of quantification (2.5 µl)) therefore no analysis was scheduled.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Species: Daphnia magna (Straus)
Age: Daphnia magna, not older than 24 hours.
The strain with the parent generation was bred and maintained in vessels containing a batch of Daphnia magna of different ages.
Preparing a new study, juvenile Daphnia magna were separated and placed in 100 ml of reconstituted water. The water was renewed and the Daphni magna were fed with a suspension of fresh water algae once a week.
Newborn animals were separated and allocated to the different dose groups.
The Daphnia magna were kept in reconstituted water in glass vessels. The study was located in an air-conditioned room in the Institute of Toxicology
Lighting was controlled by a timer to provide a 16 hours light - 8 hours dark regime.
Temperature and humidity in the experimental room were measured using a thermohygrograph.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
not applicable

Test conditions

Hardness:
250 mg/L expressed as CaCO3
Test temperature:
During the experimental part, the temperature was registered in a control vessel with an electronic thermometer containing a maximum and minimum memory display: 22°C
pH:
The pH-values were measured in the control and all test material concentrations at the beginning and at the end of the experimental part.
7.9 ± 0.3
Dissolved oxygen:
The dissolved oxygen concentration (02) were measured in the control and all test material concentrations at the beginning and at the end of the experimental part.
Start of the experimental part (t=0): 97.8% ( control) and 94.4% (test item)
End of the experimental part: 96.8% (control) and 95.9% (test item)
Salinity:
no data
Nominal and measured concentrations:
Nominal concentration: 0.1 g/L
Details on test conditions:
The test medium (reconstituted water and test material) was freshly prepared. Therefore, the calibrated flask with test material and vehicle, reconstituted water, was treated in an ultrasonic device for 1 hour. Subsequently the preparation was aerated, and stirred with a magnetic stirrer for further 23 hours. After 24 hours the preparation was given through a nutsch filter (pore size >10 - <16µm). The filtrate of the test material preparation was used for the study.
20 daphnia magna were used per test material concentration and 20 per control.
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 0.1 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 0.1 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 0.1 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
In the control and in an aqueous solution of the test material concentration of nominal 0.1 g/L (limit test), no immobilized test animals were observed during the test period of 48 hours in a static open test system.
No remarkable observations were made concerning the appearance of the solution of the test material preparation. The test medium was a clear preparation and stayed unchanged throughout the study.
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under the given experimental conditions, the test material (Tin (IV) oxide) showed a nominal 24 h and 48 h EC50 value> 0.1 g/L. Thus the EC50 of Tin dioxide exceeds the water solubility.
The no effect concentration for Daphnia magna was 0.1 g/L.