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Environmental fate & pathways

Additional information on environmental fate and behaviour

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Administrative data

Endpoint:
additional information on environmental fate and behaviour
Remarks:
Essentiality of iron
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Cross-reference
Reason / purpose for cross-reference:
data waiving: supporting information
Reference
Endpoint:
bioaccumulation in aquatic species: fish
Data waiving:
other justification
Justification for data waiving:
other:
Justification for type of information:
The full list of bibliographical references cited in the justification for data waiving is provided in the Endpoint Summary of Section 5.3.1. When appropriate, Endpoint Study Records were created in the relevant IUCLID sections to reflect these bibliographical data (see the field "Cross-references" below).
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information

Data source

Reference
Reference Type:
publication
Title:
Novel insights into iron regulation and requirement in marine medaka Oryzias melastigma
Author:
Wang & Wang
Year:
2016
Bibliographic source:
Scientific Reports 6: 26615.

Materials and methods

Principles of method if other than guideline:
The study systematically investigated the Fe regulation and requirement in marine medaka. Briefly, the critical Fe requirements at different life stages (i.e., larval and female adult) were identified through whole body Fe content screen. A conventional 2-week feeding experiment was subsequently conducted to investigate their Fe regulation and requirement. Fe regulation was studied at both biokinetic and transcriptional levels at different time intervals over 2-week feeding experiment. Stage-dependent Fe requirement was determined based on these responses and quantified using universal applicable net Fe flux.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Iron trichloride
EC Number:
231-729-4
EC Name:
Iron trichloride
Cas Number:
7705-08-0
Molecular formula:
FeCl3
IUPAC Name:
trichloroiron
Details on test material:
FeCl3.6H2O (> 99%, Sigma Aldrich).
Specific details on test material used for the study:
Fe regulation and requirement in marine medaka were investigated by supplementing diets with FeCl3.6H2O (> 99%, Sigma Aldrich).

Results and discussion

Any other information on results incl. tables

Iron (Fe) is an essential trace element for marine fish. However, knowledge of Fe requirements at different development stages of marine fish is still limited. The publication reported the efficient Fe absorption strategies adopted by larval fish under different dietary Fe supplementary levels (i.e., 0–640 mg/kg). Biokinetically, the larval fish controlled their dietary Fe assimilation efficiency (AE, 1.6–18.5%), and enhanced their waterborne Fe uptake (ca. 2.5 fold change of uptake rate constant) once the dietary Fe was deficient (i.e., 27.4 mg Fe/kg feed). Transcriptionally, the expression of hepcidin1 (hep1; Fe regulator; i.e., 2.3–15.7 fold change) in larval fish was positively correlated with the Fe supplementary levels. Comparatively, the female adult fish were poor in assimilating the added Fe source (i.e., ferric form) with similar life-sustainable levels of Fe (i.e., 0.046–0.12 μg/g/d assimilated for Fe supplementary levels of 27.4, 162 and 657 mg Fe/kg feed).

Applicant's summary and conclusion

Executive summary:

This publication contains data on the essentiality of iron and its regulation; which is part of the arguments used to waive the information requirement in Section 5.3.1 (Bioacumulation: aquatic / sediment).