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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jan 18, 2016 - Jun 02, 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Remarks:
no OECD GLP
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
yes
Remarks:
Acetone
Details on preparation and application of test substrate:
A defined artificial soil of the following composition was used for this assay.

Sphagnum peat 10% (w/w)
Kaolinite clay(Sigma Aldrich) 20 % (w/w)
Silica sand(screened with 0.2mm sieve) 70 % (w/w)


Based on the results of preliminary test, the test substance was insoluble in water, but could be dissolved in acetone. The test substance was added into the test system by test substance solvent stock solution.
An aliquot solvent solution was separately added to certain amount of silica sand and the solvent was allowed to evaporate off prior to incorporation into formulated artificial soil with deionised reverse osmosis water. These were mixed by mixer to give blank control, solvent control, and 10, 100, and 1000 mg/kg respectively with a nominal moisture content of 40-60% by mass of the total water-holding capacity with the deionized water. Test medium preparation process of blank control group was the same with other groups, but without organic solvent and test substance. Test medium preparation process of solvent control was the same with other groups, but without test substance.
Test organisms (species):
Eisenia fetida
Details on test organisms:
TEST ORGANISM
- Common name: Eisenia fetida
- Source: Jiang Su province Jurong Stock Farm. (ID: EF-MKT-150915)
- Age at test initiation (mean and range, SD): no data
- Weight at test initiation (mean and range, SD): (572.25 +/- 6.07) mg

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): yes
- Health during acclimation (any mortality observed): no mortality, no other findings
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d
Test temperature:
20.8 - 21.2 °C
pH:
6.28 & 6.48 (start)
6.28 & 6.45 (end)
Moisture:
26.70-29.30 % (start)
26.31-28.93 % (end)
Details on test conditions:
TEST SYSTEM
- Test container (material, size): 1 L glass vessel, closed
- Amount of soil or substrate: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 4



OTHER TEST CONDITIONS
- Photoperiod: 24 h/day
- Light intensity: 542 - 589 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
day 7 and 14,

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline:
- Range finding study: 0, 10, 100, 1000 mg/kg dry soil weight
- Test concentrations in definitive study: blank, solbent, 62.5, 125, 250, 500, 1000 mg/kg dry soil weight
- Results used to determine the conditions for the definitive study: range finder results
day 7: 10 % mortality @ 1000 mg/kg
day 7: 30 % mortality @ 1000 mg/kg
Nominal and measured concentrations:
1000 mg/kg soil
Reference substance (positive control):
yes
Remarks:
Chloroacetamide
Key result
Duration:
7 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
14 d
Dose descriptor:
LD50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The mortalities of the earthworm in the blank control group and solvent control group were 2.5% and 0% respectively, which were not more than 10 per cent, so the test was valid. The mortalities of the treatment group with the norminal concentration of 1000 mg/kg dry artificial soil weight after 7 and 14 days were 7.5% and 12.5% respectively. Under condition of the test, the 7-Day LC50 and the 14-Day LC50 for the test substance to earthworms (Eisenia fetida) based on the nominal test concentrations were both higher than 1000 mg/kg dry soil weight.
Results with reference substance (positive control):
LD50 at about 15 mg/kg soil dw
Reported statistics and error estimates:
Standard statistical methods have been applied for data processing.
Validity criteria fulfilled:
yes
Conclusions:
Under conditions of the test, the 7 -day LD50 and the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 1000 mg/kg dry soil weight.

Description of key information

OECD 207: LC50 > 1000 mg/kg dry soil weight

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
1 000 mg/kg soil dw

Additional information