A mixture of cis- and trans-cyclohexadec-8-en-1-one

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987-11-17 to 1987-11-21

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Supposed as the informatiom was not reported.

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

GC-analysis

Details on sampling:

A sub-sample (250 ml) of the test solution was measured into a separating funnel and extracted with dichloromethane (2 x 75 ml). The extracts were filtered through anhydrous sodium sulphate and combined in a round bottomed flask. The solvent was evaporated under vacuum just to dryness (RFE at 35 °C) and the residue dissolved in an appropriate volume of carbon tetrachloride (4-30 ml).

Vehicle:

yes

Remarks:

Tween 80-acetone

Details on test solutions:

Preliminary solution in 10% Tween 80-acetone.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Strain: Salmo gairdneri
- Source: Parkwood Trout Farm, Kent.
- Length at study initiation (length definition, mean, range and SD): 4.2 cm (S.D. = 0.4 cm)
- Weight at study initiation (mean and range, SD): 1.38 g (S.D. = 0.37 g)

ACCLIMATION
- Acclimation period: Stock held since 9 October 1986 and acclimatised to test conditions from 3'November 1986.
- Acclimation conditions (same as test or not): yes
- Type and amount of food during acclimation: Commercial trout pellets, daily. Discontinued 24 h prior to test
- Health during acclimation (any mortality observed): 0%

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

350 mg/L as CaCO3 (This value is slightly higher than the recommended Tange but is not considered to have had any significant effects an the results of the test).

Test temperature:

13 +/- 1 °C

pH:

7.4 - 7.8

Dissolved oxygen:

9.6 -10.7 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0.56, 1.0, 1.8, 3.2 and 5.6 mg/L;
Mean measured concentrations:
at 0 h: 0.597, 1.06, 1.79, 3.101 and 5.374 mg/L;
at 24 h: 0.071, 0.207, 0.600, 1.787 and 3.353 mg/L;
at 96 h: 0.06 and 0.309 mg/L (only for the nominal concentraion 0.56 and 1.0 mg/L)

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria
- Fill volume: 20 L
- Aeration: Gentle aeration via narrow bore glass tubes.
- Renewal rate of test solution (frequency): 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.69 g/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.75 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

See below

Sublethal observations / clinical signs:

Cumulative mortality data for rainbow trout exposed for 96 h to test item:

Concentration [mg/L]	Cumulative mortality (initial population : 10)
	3 h	6 h	24 h	48 h	72 h	96 h
Control	0	0	0	0	0	0
0.56	0	0	0	1	1	1
1.0	1	1	1	5	6	9
1.8	1	8	10	10	10	10
3.2	3	9	10	10	10	10
5.6	8	10	10	10	10	10

Validity criteria fulfilled:

yes

Conclusions:

The value of LC50 (96 h) of the test item to fish was determined to be 0.75 mg/L based on the nominal concentration.

Executive summary:

The acute biological effect of the item on fish was performed with Rainbow trout according to OECD TG 203 (1984) and GLP. The test was conducted under semi-static conditions with 5 concentrations: 0.56, 1.0, 1.8, 3.2 and 5.6 mg/L with a spacing factor of 1.8. The preliminary solution was prepared in 10% Tween 80-acetone. The concentrations were measured via GC-method. The value of LC50 (96 h) of the test item to fish was determined to be 0.75 mg/L based on the nominal concentration.

Description of key information

A study on the acute toxicity of the item to fish is available. The test was performed with Rainbow trout according to OECD TG 203 (1984) and GLP under semi-static conditions with 5 concentrations: 0.56, 1.0, 1.8, 3.2 and 5.6 mg/L with a spacing factor of 1.8. The preliminary solution was prepared in 10% Tween 80-acetone. The concentrations were measured via GC-method. The value of LC50 (96 h) of the test item to fish was determined to be 0.75 mg/L based on the nominal concentration.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

0.75 mg/L