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EC number: 241-749-5 | CAS number: 17766-26-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
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- Specific investigations
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- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 Oct - 12 Nov 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study, well documented
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- for some strains positive controls were only included in assays with S9, for other strains positive controls were only included in assays without S9 mix.
- Principles of method if other than guideline:
- No reference to the overall method used is given in the study report. However, the method used is well described and references for certain work steps are stated. In principal the method used is similar to OECD 471.
- GLP compliance:
- no
- Remarks:
- study performed prior to implementation of GLP
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione, trisodium salt
- EC Number:
- 241-749-5
- EC Name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione, trisodium salt
- Cas Number:
- 17766-26-6
- Molecular formula:
- C3N3S3.3Na
- IUPAC Name:
- trisodium trisulfanylidene-1,3,5-triazinane-1,3,5-triide
Constituent 1
Method
- Target gene:
- his operon (S. typhimurium strains)
tryptophan operon (E. coli strain)
Species / strainopen allclose all
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 100, TA 1537, TA 1538, TA 98
- Species / strain / cell type:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9 mix) prepared from the liver of male rats treated with Aroclor 1254 (500 mg/kg bw)
- Test concentrations with justification for top dose:
- 378, 1130, 3780, 11300, 37800 and 113300 µg TMT15/plate (equivalent to 56.7, 169.5, 567, 1695, 5670, 16995 µg anhydrous TMT/plate)
- Vehicle / solvent:
- - Vehicle/solvent used: distilled water
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- distilled water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: +S9: pyrene (400µg/plate in DMSO, TA1537); 4-acetylaminofluorene (1mg/plate in DMSO, TA1538, TA98); -S9: sodium azide (1µg/plate in water, TA1535); methyl methanesulphonate (100µg/plate in DMSO, TA100, E.coli); 9-aminoacridine (50 µg/plate in DMSO,TA1537)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: 1st and 2nd main experiment: plate incorporation method
DURATION
- Exposure duration: 48 hours (37°C)
NUMBER OF REPLICATIONS: triplicates in two independent experiments
DETERMINATION OF CYTOTOXICITY: reduction in the total number of bacteria added to the plate (i.e. thin background lawn) - Evaluation criteria:
- The test substance may be considered positive , if the following criteria are met:
S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and E. coli at least a doubling of the mean control values
S. typhimurium TA 100 at least a 1.5-fold increase over control value
In addition, dose-related responses were considered. - Statistics:
- Mean values were calculated.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: S. typhimurium TA 1535, TA 100, TA 1537, TA 1538, TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- slightly toxic at highest dose level
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- slightly toxic at highest dose level
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES:
The dose levels were selected on the basis of observations from a preliminary toxicity study in strain TA 100. Concentrations of 0.378, 1.13, 3.78, 11.3, 37.8 and 113.3 mg/plate were not toxic in strain TA 100 as defined by an observed reduction in the total number of bacteria (i.e. thin background lawn) . - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
Under the conditions of this study, the substance was not mutagenic in any of the 6 strains tested.
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