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EC number: 200-296-3 | CAS number: 56-89-3
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- L-cysteine
- EC Number:
- 200-158-2
- EC Name:
- L-cysteine
- Cas Number:
- 52-90-4
- Molecular formula:
- C3H7NO2S
- IUPAC Name:
- L-cysteine
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Name: L-Cysteine
Chemical Name: L-( +)-Cysteine
CAS No.: 52-90-4
Batch No.: 12030506
Physical State: solid
Colour: white
Molecular Weight: 121.1
pH: 4.5-6
Storage Conditions: 2-8°C, protected from light
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-Mix
- Test concentrations with justification for top dose:
- Experiment I:
without and with metabolic activation: 2.5, 5.0 and 10.0 mM
Experiment II:
without metabolic activation: 1.25, 2.5 and 5.0 mM
with metabolic activation: 2.5, 5.0 and 10.0 mM - Vehicle / solvent:
- Complete Culture Medium
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- ethylmethanesulphonate
- Remarks:
- EPA without metabolic activation, CPA with metabolic activation
- Details on test system and experimental conditions:
- A pre-experiment was conducted under identical conditions as described for the main experiment. The following concentrations were tested without and with S9 mix: 0.020, 0.039, 0.078, 0.16, 0.31, 0.63, 1.25, 2.5, 5.0 and 10.0 mM.
The cultures were treated at each concentration. The selection of the concentrations used in experiments I and II was based on data from the pre-experiment. The following concentrations were used in the main experiments:
Experiment I and II:
without and with metabolic activation: 0.31, 0.63, 1.25, 2.5, 5.0 and 10.0 mM
In experiment I the cells were treated with the test item for 4 h without and with metabolic activation. The metaphases were prepared 20 h after the treatment. In experiment II, with metabolic activation, the cells were treated for 4 h and the metaphases were prepared 20 h after the treatment. In experiment II, without metabolic activation, the cells were treated for 20 h and the metaphases were prepared at the end of the treatment. The dose group selection for microscopic analyses of chromosomal aberrations was based on the mitotic index in accordance with the guidelines.
The following concentrations were selected in the main experiments for the microscopic analyses:
Experiment I: without and with metabolic activation: 2.5, 5.0 and 10.0 mM
Experiment II: without metabolic activation: 1.25, 2.5 and 5.0 mM; with metabolic activation: 2.5, 5.0 and 10.0 mM
Exposure time 4 hours (without and with S9 mix; first experiment with S9 mix;
second experiment):
Two days after seeding of the cells, the culture medium was replaced with serumfree medium containing the test item and S9 mix (with metabolic activation). Additional negative and positive controls were performed without and with exogenic metabolic activation.
4 h after treatment the cultures were washed twice with PBS and cultured in complete medium for the remaining culture time.
Exposure time 20 hours (without S9 mix; second experiment):
Two days after seeding of the cells the culture medium is replaced with complete medium containing the test item. This medium is not changed until preparation of the cells. The cells were prepared at the end of the incubation. Additional negative and positive controls were tested.
All cultures were incubated at 37 ± 1 °C in a humidified atmosphere with 5.0% CO2 (95.0% air).
Preparation of the Cultures
Colcemid® was added to the cultures (0.2 μg/mL culture medium) 17.5 hours after the start of each treatment (4 h and 20 h treatment). 2.5 h later, the cells were treated on the slides in the chambers with hypotonic solution (0.4% KCl) for 20 min at 37 °C. After incubation in the hypotonic solution the cells were fixed with 3 + 1 methanol + glacial acetic acid (v/v). All the steps were carried out on precision hot plates at 37 °C. After fixation step the slides were air dried and stained with Giemsa. - Evaluation criteria:
- The chromosomal aberration assayis considered acceptable if it meets the following criteria:
- the number of aberrations found in the negative and/or solvent controls falls within the range of historical laboratory control data: 0.0% - 4.0% (without and with metabolic activation),
- the positive control substance should produce biologically relevant increases in the number of cells with structural chromosome aberrations.
A test item is considered to be negative if there is no biologically relevant increase in the percentages of aberrant cells above concurrent control levels, at any dose group. Although most experiments will give clearly positive or negative results, in some cases the data set will preclude making a definitive judgement about the activity of the test substance.
Results and discussion
Test results
- Species / strain:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test item L-Cysteine is considered to be non-clastogenic in this chromosome aberration test. - Executive summary:
To investigate the potential of L-Cysteine (99.6% pure) to induce structural chromosome aberrations in Chinese hamster V79 cells, an in vitro chromosome aberration assay according to OECD Guideline 473 was carried out.
The metaphases were prepared 20 h after start of treatment with the test item. The treatment interval was 4 hwithoutandwithmetabolic activation in experiment I. In experiment II, the treatment interval was 20 hwithoutand 4 hwithmetabolic activation. Parallel cultures were treated at each concentration. 100 metaphases per culture were scored for structural chromosomal aberrations.
The following concentrations were evaluated for the microscopic analysis of chromosomal aberrations:
Experiment I:withoutandwithmetabolic activation: 2.5, 5.0 and 10.0 mM
Experiment II:withoutmetabolic activation: 1.25, 2.5 and 5.0 mM;withmetabolic activation: 2.5, 5.0 and 10.0 mM
No precipitation of the test item was notedwithoutandwithmetabolic activation in all dose groups evaluated in experiment I and II. No cytotoxic effects of the test item were notedwithoutandwithmetabolic activation in all concentrations evaluated in experiment I and II. In experiment Iwithoutandwithmetabolic activation no biologically relevant increase of the aberration rates was noted after treatment with the test item. The aberration rates of all concentrations treated with the test item were within the historical control data of the negative control.
In experiment IIwithoutmetabolic activation a slight increase of aberrant cells (4.5%) was noted compared to the historical control data (0.0% - 4.0%). However, as there was no concentration relationship observed and only one of the four evaluated slides displayed an increase (2%, 1%, 3% and 12% aberrant cells observed) this effect was considered as not biologically relevant.
In experiment IIwithmetabolic activation no biologically relevant increase of the aberration rates was noted after treatment with the test item. In the experiments I and IIwithoutandwithmetabolic activation no biologically relevant increase in the frequencies of polyploid cells was found after treatment with the test item as compared to the negative controls.
In the experiments I and IIwithoutandwithmetabolic activation no biologically relevant increase in the frequencies of polyploid cells was found after treatment with the test item as compared to the negative controls.
EMS (400 and 900μg/mL) and CPA (0.83μg/mL) were used as positive controls and induced distinct and biologically relevant increases in cells with structural chromosomal aberrations, thus proving the efficiency of the test system to indicate potential clastogenic effects. In the first experimentwithoutmetabolic activation the positive control EMS (900μg/mL) displayed a percentage of 5% aberrant cells.
However, on one of the two slides additional five aberrations were seen in metaphases with less than 21 chromosomes. This would lead to an aberration rate of 7.5%.
In conclusion, it can be stated that during the described in vitro chromosome aberration test and under the experimental conditions reported, the test item LCysteine did not induce structural chromosomal aberrations in the V79 Chinese hamster cell line. Therefore, the test item L-Cysteine is considered to be non-clastogenic in this chromosome aberration test.
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