Registration Dossier
Registration Dossier
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EC number: 932-019-3 | CAS number: -
Biodegradation in water and sediment: simulation tests
Administrative data
- Endpoint:
- biodegradation in water: sewage treatment simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 303 B (Simulation Test - Aerobic Sewage Treatment. B. Biofilms)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ascorbic acid
- EC Number:
- 200-066-2
- EC Name:
- Ascorbic acid
- Cas Number:
- 50-81-7
- Molecular formula:
- C6H8O6
- IUPAC Name:
- 5-(1,2-dihydroxyethyl)-3,4-dihydroxyfuran-2(5H)-one (non-preferred name)
Constituent 1
- Radiolabelling:
- no
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on source and properties of surface water:
- None given.
- Details on source and properties of sediment:
- The activated sludge was exposed under aerobic conditions to 733 mg/l L-ascorbic acid and was monitored for 15 days.
- Duration of test (contact time):
- 15 d
Initial test substance concentration
- Initial conc.:
- 733 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
Results and discussion
% Degradation
- Key result
- % Degr.:
- 97
- Parameter:
- DOC removal
- Sampling time:
- 5 d
- Transformation products:
- not measured
Any other information on results incl. tables
Test Results:
Day |
% Deg. Test 1 |
% Deg. Test 2 |
Mean % Deg. |
1 |
48 |
57 |
52.5 |
5 |
97 |
97 |
97 |
7 |
98 |
97 |
97.5 |
9 |
98 |
99 |
98.5 |
12 |
99 |
99 |
99 |
15 |
100 |
100 |
100 |
Applicant's summary and conclusion
- Conclusions:
- L-ascorbic acid shows 97% biodegradability after 5 days and 100% biodegradablility after 15 days.
- Executive summary:
The aerobic biodegradability of L-ascorbic acid was tested using the modified Zahn-Wellens method. A mixture of activated sludge was exposed to 733 mg/l L-ascorbic acid and it was monitored for 15 days to determine the DOC values. The % biodegradation was 97% after 5 days and 100% after 15 days. Therefore, the substance is fully biodegradable.
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