1H-Pyrazolo[3,4-c]pyridine-3-carboxylic acid, 4,5,6,7-tetrahydro-1-(4-methoxyphenyl)-6-(4-nitrophenyl)-7-oxo-, ethyl ester

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental Starting Date: 25 April 2014 Experimental Completion Date: 03 June 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Remarks:

Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results. The study report was conclusive, done to a valid guideline and the study was conducted under GLP conditions.

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Deviations:

no

GLP compliance:

yes

Type of method:

pycnometer method

Type:

relative density

Density:

1.4

Temp.:

22.8 °C

Results

Calibration

The pressure readings and the calculated volume for the reference and sample cells are shown in the following table:

Table 3.6

 

Determination	P1 (psig)	P2 (psig)	P3 (psig)	P4 (psig)	Reference Cell Volume (cm^3)	Sample Cell Volume (cm^3)
1	17.138	6.483	17.440	8.634	90.696	149.062
2	17.319	6.552	17.301	8.569	90.597	148.879
3	17.150	6.488	17.406	8.619	90.661	148.987

 

Mean reference cell volume   :          90.652 cm³

Mean sample cell volume       :          148.976 cm³

 

As the reference cell volume and sample cell volume were within the defined tolerance (90.953 ± 1.0 cm³and 149.225 ± 1.0 cm³for the reference and sample cell volumes respectively) the gas comparison pycnometer was therefore considered acceptable for use.

 

Sample

The pressure readings, calculated volumes and density values obtained for the test item are shown in the following table:

Table 3.7

 

Determination	Test	P1(sample) (psig)	P2(sample) (psig)	Volume (cm^3)	Density (kg/m^3)	Mean Density (kg/m^3)
1	A	17.049	7.432	31.673	1.40 x 10^3	1.40 x 10^3
	B	17.082	7.448	31.718	1.40 x 10^3
	C	17.206	7.502	31.717	1.40 x 10^3
2	A	17.320	7.682	35.243	1.39 x 10^3	1.40 x 10^3
	B	17.277	7.661	35.192	1.40 x 10^3
	C	17.301	7.670	35.148	1.40 x 10^3

 

Mean density             :          1.40 x 10³ kg/m³

Relative density         :          1.40

Temperature               :          22.8 ± 0.5 °C

Conclusions:

The density of the test item has been determined to be 1.40 x 103kg/m3at 22.8 ± 0.5 °C, relative density 1.40.

Executive summary:

The determination was carried with a gas comparison pycnometer, using a procedure designed to be compatible with Method A3 Relative Density of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 109 of the OECD Guidelines for Testing of Chemicals, 02 October 2012.

Instrument                  :          Gas comparison pycnometer (Multi-Pycnometer, Quantachrome, Syosset, N.Y., USA).

Calibration Sphere(s) :          A stainless steel sphere with a calibrated volume (V_cal= 56.559).

 

Gas                             :          Helium.

 

Conditions                 :          All determinations were performed at 22.8 ± 0.5 °C.

Conclusion

The density of the test item has been determined to be 1.40 x 10³kg/m³at 22.8 ± 0.5 °C, relative density 1.40.

Description of key information

The determination was carried with a gas comparison pycnometer, using a procedure designed to be compatible with Method A3 Relative Density of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 109 of the OECD Guidelines for Testing of Chemicals, 02 October 2012.

Conclusion

The density of the test item has been determined to be 1.40 x 10³kg/m³at 22.8 ± 0.5 °C, relative density 1.40.

Key value for chemical safety assessment

Relative density at 20C:

1.4