3,4-dimethoxyphenylacetonitrile

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure accordint to German national standard methods (DIN), no GLP, no analytical monitoring.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 part L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dimethoxyphenylacetonitrile

EC Number:

202-225-1

EC Name:

3,4-dimethoxyphenylacetonitrile

Cas Number:

93-17-4

Molecular formula:

C10H11NO2

IUPAC Name:

3,4-dimethoxyphenylacetonitrile

Details on test material:

- Name of test material (as cited in study report): 3,4-dimethoxyphenylacetonitrile (Veratrylcyanid krist. SF9)
- Lot/batch No.: 22078
- Analytical purity: 98.5 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment. Subsequently the fish were placed into the test aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenweststedt, Germany
- Length at study initiation (length definition, mean, range and SD): 6.7 cm (range 5.7 - 7.2 cm)
- Weight at study initiation (mean and range, SD): 2.9 g (range 1.5 - 4.4 g)


ACCLIMATION
- Acclimation period: 3 days
- Acclimation conditions (same as test or not):
- Type and amount of food: Growing feed F/B 5 0, Ssniff Spezialdiaeten GmbH, Soest, Germany; ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

21 °C

pH:

7.3 - 7.9

Dissolved oxygen:

6.4 - 8.8 mg/l

Nominal and measured concentrations:

Nominal test concentrations:
10.0, 21.5, 46.4, 100, 215*, 464* mg/l.


Since no LC100 was observed in any of the test concentrations up to 100 mg/l, 2 additional test concentrations 215 and 464 mg/l were added to the test, one week after test start.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 2.9 g fish/l test water

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982.
- Ca/mg ratio: 4:1
- Conductivity: max . 10 µS

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 0, 24, 48, 72 and 96 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Results used to determine the conditions for the definitive study: LC50 after 96 hours 10 - 100 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Undissolved test substance was visible, increasing with an increase in the concentration.

Any other information on results incl. tables

Sublethal observations / clinical signs:

During the 96 h exposure to Test substance, mortalities were as follows:

Nominal test / Cumulative mortality (%) after x hours
concentration / 1 / 4 / 24 / 48 / 72 / 96 /
(mg/l)
Control / 0 / 0 / 0 / 0 / 0 / 0 /
10 / 0 / 0 / 0 / 0 / 0 / 0 /
21.5 / 0 / 0 / 0 / 0 / 0 / 0 /
46.4 / 0 / 0 / 0 / 0 / 0 / 0 /
100.0 / 0 / 0 / 10 / 40 / 40 / 40 /
215.0 / 0 / 100 / 100 / 100 / 100 / 100 /
464.0 / 100 / 100 / 100 / 100 / 100 / 100 /


Symptoms of intoxication:

Nominal test / Symptoms of intoxication after x hours
concentration / 1 / 4 / 24 / 48 / 72 / 96 /
(mg/l)
Control / - / - / - / - / - / - /
10 / - / - / - / - / - / - /
21.5 / - / - / - / - / - / - /
46.4 / - / - / - / - / - / - /
100.0 / - / T / LN / L / L / L /
215.0 / AN / + / + / + / + / + /
464.0 / + / + / + / + / + / + /


Definitions of Symptoms:
-: No symptoms
+: All fish dead
A: Apathy
N: Narcotic-like state
L: Gasping
T: Tumbling